Growth cone collapse through coincident loss of actin bundles and leading edge actin without actin depolymerization.
Growth cone collapse through coincident loss of actin bundles and leading edge actin without actin depolymerization.
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DOI：
10.1083/jcb.153.5.1071
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发表时间：
2001-05-28
期刊：
影响因子：
--
通讯作者：
Cohan CS
中科院分区：
文献类型：
--
作者：
Zhou FQ;Cohan CS
排斥的引导线索可以使整个生长锥体塌陷以阻止轴突生长，也可以导致不对称塌陷导致生长锥体转动。生长锥体是如何通过重新排列细胞骨架将排斥信号转化为这种形态变化的，目前还不清楚。我们研究了三个因素，包括5-羟色胺、肌球蛋白轻链激酶抑制剂和佛波酯，能够在条件培养液中诱导伸展的Helisoma生长锥体崩溃。为了研究导致塌陷的细胞骨架事件，我们在聚赖氨酸上培养了Helisoma生长锥，其中片脂塌陷通过底物粘合来防止。我们发现，导致延伸的生长锥崩溃的所有三个因素也都导致多赖氨酸附着的生长锥中的肌动蛋白束丢失，而不损失肌动蛋白网络。此外，肌动蛋白束丢失与特定的丝状肌动蛋白重新分布远离前沿有关，这是排斥因素的特征。最后，我们使用延时研究提供了直接证据，证明肌动蛋白束丢失与崩溃平行延伸生长锥体。综上所述，这些结果表明，肌动蛋白束可能是各种塌陷因子共同的细胞骨架靶标，这些因子可能使用不同的信号通路汇聚在一起，导致生长锥体塌陷。
Repulsive guidance cues can either collapse the whole growth cone to arrest neurite outgrowth or cause asymmetric collapse leading to growth cone turning. How signals from repulsive cues are translated by growth cones into this morphological change through rearranging the cytoskeleton is unclear. We examined three factors that are able to induce the collapse of extending Helisoma growth cones in conditioned medium, including serotonin, myosin light chain kinase inhibitor, and phorbol ester. To study the cytoskeletal events contributing to collapse, we cultured Helisoma growth cones on polylysine in which lamellipodial collapse was prevented by substrate adhesion. We found that all three factors that induced collapse of extending growth cones also caused actin bundle loss in polylysine-attached growth cones without loss of actin meshwork. In addition, actin bundle loss correlated with specific filamentous actin redistribution away from the leading edge that is characteristic of repulsive factors. Finally, we provide direct evidence using time-lapse studies of extending growth cones that actin bundle loss paralleled collapse. Taken together, these results suggest that actin bundles could be a common cytoskeletal target of various collapsing factors, which may use different signaling pathways that converge to induce growth cone collapse.
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影响因子：
5.7
作者：
Bentley, David;O'Connor, Timothy P.
通讯作者：
O'Connor, Timothy P.
影响因子：
56.9
作者：
HAYDON, PG;MCCOBB, DP;KATER, SB
通讯作者：
KATER, SB
DOI：
10.1083/jcb.121.4.867
发表时间：
1993-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Fan J;Mansfield SG;Redmond T;Gordon-Weeks PR;Raper JA
通讯作者：
Raper JA
DOI：
10.1002/neu.480270409
发表时间：
1995-08-01
期刊：
JOURNAL OF NEUROBIOLOGY
影响因子：
--
作者：
GOLDBERG, DJ;WU, DY
通讯作者：
WU, DY
影响因子：
4.8
作者：
Amano, M;Ito, M;Kaibuchi, K
通讯作者：
Kaibuchi, K