Pulmonary vessel casting in a rat model of monocrotaline-mediated pulmonary hypertension
Pulmonary vessel casting in a rat model of monocrotaline-mediated pulmonary hypertension
复制标题
野百合碱介导的肺动脉高压大鼠模型的肺血管铸型
DOI：
10.1177/2045894020922129
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发表时间：
2020-07
影响因子：
2.6
通讯作者：
Chen Jiwang
中科院分区：
文献类型：
--
作者：
Zhu Zhongkai;Wang Yifan;Long Amy;Feng Tianyu;Ocampo Maria;Chen Sunny;Tang Haiyang;Guo Qiang;Minshall Richard;Makino Ayako;Huang Wei;Chen Jiwang
肺动脉高压是一种以肺血管收缩和肺动脉重塑为特征的慢性血管疾病。肺动脉重构主要是由于小肺动脉壁增厚和管腔闭塞。以前的研究已经使用组织病理学描述了肺切片的血管内变化，但很少有人能够获得肺血管系统的精细图像。在这项研究中，我们使用 Microfil 化合物在野百合碱诱导的肺动脉高压大鼠模型中铸造肺动脉。该模型展示了能够根据血管分叉/接合处的数量对远端肺动脉分支进行量化的高质量图像。与对照组相比，野百合碱组远端肺动脉的分支和连接处计数显着减少，并且这种效应与各组观察到的平均肺动脉压成反比。提出了肺血管的模式和肺血管铸型的方法，为肺动脉高压和其他涉及血管重塑的肺部疾病引起的肺动脉重塑的未来研究提供基础。
Pulmonary hypertension is a chronic vascular disease characterized by pulmonary vasoconstriction and pulmonary arterial remodeling. Pulmonary arterial remodeling is mainly due to small pulmonary arterial wall thickening and lumen occlusion. Previous studies have described intravascular changes in lung sections using histopathology, but few were able to obtain a fine detailed image of the pulmonary vascular system. In this study, we used Microfil compounds to cast the pulmonary arteries in a rat model of monocrotaline-induced pulmonary hypertension. High-quality images that enabled quantification of distal pulmonary arterial branching based on the number of vessel bifurcations/junctions were demonstrated in this model. The branch and junction counts of distal pulmonary arteries significantly decreased in the monocrotaline group compared to the control group, and this effect was inversely proportional to the mean pulmonary artery pressure observed in each group. The patterns of pulmonary vasculature and the methods for pulmonary vessel casting are presented to provide a basis for future studies of pulmonary arterial remodeling due to pulmonary hypertension and other lung diseases that involve the remodeling of vasculature.
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影响因子：
2.6
作者：
Faight EM;Verdelis K;Zourelias L;Chong R;Benza RL;Shields KJ
通讯作者：
Shields KJ
影响因子：
24.3
作者：
STREK, P;NOWOGRODZKAZAGORSKA, M;MIODONSKI, AJ
通讯作者：
MIODONSKI, AJ
影响因子：
5.8
作者：
Townsley MI
通讯作者：
Townsley MI
DOI：
10.1152/ajpheart.01097.2002
发表时间：
2003-09-01
影响因子：
4.8
作者：
Girgis, RE;Li, DC;Johns, RA
通讯作者：
Johns, RA
影响因子：
2.6
作者：
K. Shields;K. Verdelis;M. Passineau;Erin M. Faight;L. Zourelias;Changgong Wu;R. Chong;R. Benza
通讯作者：
K. Shields;K. Verdelis;M. Passineau;Erin M. Faight;L. Zourelias;Changgong Wu;R. Chong;R. Benza