Bioprocessing of soybeans (Glycine max L.) by solid-state fermentation with Eurotium cristatum YL-1 improves total phenolic content, isoflavone aglycones, and antioxidant activity.
Bioprocessing of soybeans (Glycine max L.) by solid-state fermentation with Eurotium cristatum YL-1 improves total phenolic content, isoflavone aglycones, and antioxidant activity.
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通过固态发酵的大豆（Glycine Max L.）用Eurotium cristatum YL-1对大豆的生物处理可改善总酚含量，异黄酮aglycone和抗氧化活性。
作者：
本研究以大豆（Glycine max L.）以冠突散囊菌YL-1为菌种，采用固态发酵技术对发酵液进行生物处理。研究了SSF对大豆不同发酵阶段总酚含量、多酚类物质含量及抗氧化活性的影响。结果表明，在SSF过程中，TPC和甘草苷元显著增加，而糖苷类甘草酮显著减少。经过15天的SSF，TPC，大豆苷元，染料木素，和总苷元的大豆分别约1.9，10.4，8.4，和9.4倍高，分别比那些非发酵大豆。SSF过程中β-葡萄糖苷酶活性很高，α-淀粉酶和蛋白酶活性处于中等水平，纤维素酶活性相对较低。TPC与α-淀粉酶（R2 = 0.9452）、β-葡萄糖苷酶（R2 = 0.9559）、纤维素酶（R2 = 0.9783）和蛋白酶（R2 = 0.6785）的活性呈极显著正相关。线性分析验证了E. cristatum有助于将大豆异黄酮苷生物转化为糖苷配基形式。SSF处理显著提高了大豆对DPPH自由基和ABTS stec+的清除能力、还原能力和铁还原抗氧化能力。主成分分析和Pearson相关性分析表明，大豆抗氧化能力提高的主要原因是大豆蛋白质含量和大豆苷元含量的提高。因此，我们的研究结果表明，固态生物处理与E。鸡冠状花序是提高大豆总蛋白、大豆苷元和抗氧化能力的有效途径。与非发酵大豆相比，生物加工大豆产品可能是一种富含抗氧化剂的健康食品补充剂，因此可能是天然抗氧化剂的来源。利用冠突散囊菌进行固态生物加工是提高大豆总酚含量、总苷元含量和抗氧化活性的有效途径。
In this study, soybean (Glycine max L.) was bioprocessed with fungal strain Eurotium cristatum YL-1 by using the solid-state fermentation (SSF) technique. The effect of SSF on total phenolic content (TPC), isoflavone compositions, and antioxidant activity of soybean during different fermentation periods was evaluated. Results showed that TPC and isoflavone aglycones were significantly increased, whereas glucoside isoflavones were remarkably reduced during SSF. After 15 days of SSF, the TPC, daidzein, genistein, and total aglycones of soybeans were approximately 1.9-, 10.4-, 8.4-, and 9.4-fold higher, respectively, than those of non-fermented soybeans. During SSF, β-glucosidase activity was very high, whereas α-amylase and protease activities were at moderate levels, and cellulase activity was relatively low. A highly positive correlation was found between TPC and the activities of α-amylase (correlation coefficient R2 = 0.9452), β-glucosidase (R2 = 0.9559), cellulase (R2 = 0.9783), and protease (R2 = 0.6785). Linear analysis validated that the β-glucosidase produced by E. cristatum contributed to the bioconversion of soybean isoflavone glucosides into their aglycone forms. The DPPH radical and ABTS˙+ scavenging activity, reducing power, and ferric reducing antioxidant power of soybeans were considerably enhanced during SSF. Principal component analysis and Pearson's correlation analysis verified that the improvement in TPC and isoflavone aglycone content during SSF was mainly responsible for the improved antioxidant capacity of soybeans. Thus, our results demonstrated that solid-state bioprocessing with E. cristatum is an effective approach for the enhancement of the TPC, isoflavone aglycones, and antioxidant capacity of soybeans. Bioprocessed soybean products might be a healthy food supplement rich in antioxidants compared with non-fermented soybean and thus could be a source of natural antioxidants. Solid-state bioprocessing with Eurotium cristatum is an effective approach for the enhancement of total phenolic content, isoflavone aglycones, and antioxidant activity of soybeans.
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DOI：
10.1016/j.ifset.2019.102193
发表时间：
2019-10-01
影响因子：
6.6
作者：
Aruna, T. E.
通讯作者：
Aruna, T. E.
影响因子：
3.3
作者：
Cheng, Kuan-Chen;Wu, Jiun-Yi;Liu, Wen-Hsiung
通讯作者：
Liu, Wen-Hsiung
影响因子：
4
作者：
Abd Razak, Dang Lelamurni;Abd Rashid, Nur Yuhasliza;Long, Kamariah
通讯作者：
Long, Kamariah
影响因子：
8.8
作者：
Kim, Jin-Kwang;Cui, Chang-Hao;Im, Wan-Taek
通讯作者：
Im, Wan-Taek
影响因子：
3.8
作者：
Acosta-Estrada, Beatriz A.;Villela-Castrejon, Javier;Gutierrez-Uribe, Janet A.
通讯作者：
Gutierrez-Uribe, Janet A.