Use of fluorescent probes for ROS to tease apart Type I and Type II photochemical pathways in photodynamic therapy.
Use of fluorescent probes for ROS to tease apart Type I and Type II photochemical pathways in photodynamic therapy.
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DOI：
10.1016/j.ymeth.2016.06.025
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发表时间：
2016-10-15
期刊：
影响因子：
4.8
通讯作者：
Hamblin, Michael R.
中科院分区：
文献类型：
--
作者：
Garcia-Diaz, Maria;Huang, Ying-Ying;Hamblin, Michael R.
关键词：
这是一份未经编辑的手稿的PDF文件，已被接受出版。为了向我们的客户提供服务，我们提供这份手稿的早期版本。手稿将经过复制编辑、排版和对最终校样的审查，然后以其最终可引用的形式出版。请注意，在制作过程中，可能会发现可能影响内容的错误，适用于该期刊的所有法律免责声明均适用。光动力疗法包括用无害的可见光激发一种无毒的染料，产生一种长寿命的三重态，这种三态可以与分子氧相互作用产生活性氧物种(ROS)，从而破坏生物分子并杀死细胞。电子转移产生的ROS(类型1)包括超氧化物、过氧化氢和羟基自由基(HO·)，而单线态氧(1O2)是通过能量转移产生的。有不同的方法来区分这两种途径，其中一些比另一些更具体或更敏感。在这篇综述中，我们介绍了两种荧光探针的使用：单线态氧传感器绿(SOSG)检测1O2；4-羟基苯基荧光素(HPF)检测HO·。收集了一些有趣的数据，涉及到功能化富勒烯与四吡咯的复合、稳定的合成细菌素与中心金属和不含中心金属的合成、吩硫氮染料与叠氮等无机盐的相互作用等。
This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Photodynamic therapy involves the excitation of a non-toxic dye by harmless visible light to produce a long-lived triplet state that can interact with molecular oxygen to produce reactive oxygen species (ROS), which can damage biomolecules and kill cells. ROS produced by electron transfer (Type 1) include superoxide, hydrogen peroxide and hydroxyl radical (HO•), while singlet oxygen (1O2) is produced by energy transfer. Diverse methods exist to distinguish between these two pathways, some of which are more specific or more sensitive than others. In this review we cover the use of two fluorescence probes: singlet oxygen sensor green (SOSG) detects 1O2; and 4-hydroxyphenyl-fluorescein (HPF) that detects HO•. Interesting data was collected concerning the photochemical pathways of functionalized fullerenes compoared to tetrapyrroles, stable synthetic bacteriochlorins with and without central metals, phenothiazinium dyes interacting with inorganic salts such as azide.
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