Quantitative assessment of liver fibrosis by digital image analysis reveals correlation with qualitative clinical fibrosis staging in liver transplant patients.
Quantitative assessment of liver fibrosis by digital image analysis reveals correlation with qualitative clinical fibrosis staging in liver transplant patients.
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DOI：
10.1371/journal.pone.0239624
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发表时间：
2020
期刊：
影响因子：
3.7
通讯作者：
Farris AB
中科院分区：
文献类型：
--
作者：
Jiang K;Mohammad MK;Dar WA;Kong J;Farris AB
组织数字化技术为肝脏组织病理学研究提供了重要的定量数据。我们的目的是利用数字图像分析(DIA)通过组织病理学切片的定量形态测量来评估肝纤维化程度，并进一步调查是否存在与组织病理学评分(Scheuer分期)的相关性。收集了一项对至少两次肝移植后活检的患者进行的回顾性研究，这些患者在基线时的≤-2处于Scheuer分期。门静脉纤维化百分比(%)和大小(μm~2)用放射免疫法测定，临床纤维化评分用Scheuer系统测定。用Spearman相关分析计算DIA测量结果与Scheuer评分的相关性。通过学生t检验计算基线和复诊时平均肝纤维化水平(评分、大小和百分比)之间的差异。P值<；0.05被认为是显著的。在符合研究标准的22名患者中，54例活检用于分析。平均水平±标准误差[S.E.]门静脉纤维化百分比(%)和大小(μm2)分别从基线的46.5±3.6%和28,075±3,232μm2上升到复诊时的61.8±3.8%(t检验，P=0.005)和67,146±10,639μm2(t检验，P=0.002)。Scheuer纤维化评分的平均水平从基线的0.55±0.19上升到第二次随访时的1.14±0.26(经学生t检验，P=0.02)。门静脉纤维化百分比(%)和纤维化大小与临床肝纤维化分期直接相关，Spearman相关系数和P值分别为r=0.70P&0.0001和r=0.41P=0.002。门静脉三联体大小和纤维化百分比的数字定量评估与肝纤维化的目测组织学阶段有很强的相关性，并补充了同种异体移植监测的标准评估，提示未来WSI分析的实用价值。
Technologies for digitizing tissues provide important quantitative data for liver histopathology investigation. We aimed to assess liver fibrosis degree with quantitative morphometric measurements of histopathological sections utilizing digital image analysis (DIA) and to further investigate if a correlation with histopathologic scoring (Scheuer staging) exists. A retrospective study of patients with at least two post-liver transplant biopsies having a Scheuer stage of ≤ 2 at baseline were gathered. Portal tract fibrotic percentage (%) and size (μm2) were measured by DIA, while clinical fibrosis score was measured by the Scheuer system. Correlations between DIA measurements and Scheuer scores were computed by Spearman correlation analysis. Differences between mean levels of fibrosis (score, size, and percentage) at baseline versus second visit were computed by Student’s t-test. P values < 0.05 were considered significant. Of 22 patients who met the study criteria, 54 biopsies were included for analysis. Average levels ±standard error [S.E.] of portal tract fibrotic percentage (%) and size (μm2) progressed from 46.5 ± 3.6% at baseline to 61.8 ± 3.8% at the second visit (P = 0.005 by Student’s t-test), and from 28,075 ± 3,232 μm2 at base line to 67,146 ± 10,639 μm2 at the second visit (P = 0.002 by Student’s t-test), respectively. Average levels of Scheuer fibrosis scores progressed from 0.55±0.19 at baseline to 1.14±0.26 at the second visit (P = 0.02 by Student’s t-test). Portal tract fibrotic percentage (%) and portal tract fibrotic size were directly correlated with clinical Scheuer fibrosis stage, with Spearman correlation coefficient and P value computed as r = 0.70, P < 0.0001 and r = 0.41, P = 0.002, respectively. Digital quantitative assessment of portal triad size and fibrosis percentage demonstrates a strong correlation with visually assessed histologic stage of liver fibrosis and complements the standard assessment for allograft monitoring, suggesting the utility of future WSI analysis.
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影响因子：
25.7
作者：
Pais R;Barritt AS 4th;Calmus Y;Scatton O;Runge T;Lebray P;Poynard T;Ratziu V;Conti F
通讯作者：
Conti F
影响因子：
2.2
作者：
Almpanis Z;Demonakou M;Tiniakos D
通讯作者：
Tiniakos D
影响因子：
13.5
作者：
SCHEUER, PJ;ASHRAFZADEH, P;DUSHEIKO, GM
通讯作者：
DUSHEIKO, GM
DOI：
10.1038/s41374-020-0463-y
发表时间：
2020-10
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Roy M;Wang F;Vo H;Teng D;Teodoro G;Farris AB;Castillo-Leon E;Vos MB;Kong J
通讯作者：
Kong J
影响因子：
3.7
作者：
Axley P;Mudumbi S;Sarker S;Kuo YF;Singal AK
通讯作者：
Singal AK