Simultaneous monitoring of dopamine concentration at spatially different brain locations in vivo.
Simultaneous monitoring of dopamine concentration at spatially different brain locations in vivo.
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DOI：
10.1016/j.bios.2009.10.008
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发表时间：
2010-01-15
影响因子：
12.6
通讯作者：
McCarty GS
中科院分区：
文献类型：
--
作者：
Zachek MK;Takmakov P;Park J;Wightman RM;McCarty GS
当与微电极耦合时，背景扣除快速扫描循环伏安法（FSCV）允许在小空间位置内快速，灵敏和选择性地测定分析物。在过去的30年里，使用这种技术的实验在很大程度上局限于在单个微电极上的记录。具有紧密分离的微电极的阵列将使研究人员能够获得更多信息数据以及空间接近的探测区域。这项工作提出了第一个FSCV微电极阵列（MEA）在体内实施的能力，从不同区域的样品在空间上接近（等距1毫米内）。该阵列由熔融石英毛细管和微加工电极间隔物制成。通过同时监测电刺激多巴胺（DA）在麻醉大鼠纹状体的释放来评估阵列的功能。正如预期的那样，同时观察到不均匀的多巴胺释放。此外，雷氯必利（D2受体拮抗剂）和可卡因（单胺摄取阻滞剂）对DA释放异质性的药理学作用，在空间上不同的大脑区域，显示改变所有四个电极部位的神经递质释放。
When coupled with a microelectrode, background-subtracted fast scan cyclic voltammetry (FSCV) allows fast, sensitive and selective determination of analytes within a small spatial location. For the past 30 years experiments using this technique have been largely confined to recordings at a single microelectrode. Arrays with closely separated microelectrodes would allow researchers to gain more informative data as well as probe regions in close spatial proximity. This work presents one of the first FSCV microelectrode arrays (MEA) implemented in vivo with the ability to sample from different regions in close spatial proximity (equidistant within 1 mm). The array is manufactured from fused silica capillaries and a microfabricated electrode spacer. The functionality of the array is assessed by simultaneously monitoring electrically stimulated dopamine (DA) release in the striatum of anesthetized rat. As expected, heterogeneous dopamine release was simultaneously observed. Additionally, the pharmacological effect of raclopride (D2 receptor antagonist) and cocaine (monoamine uptake blocker) on the heterogeneity of DA release, in spatially different brain regions was shown to alter neurotransmitter release at all four electrode sites.
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影响因子：
4.7
作者：
Kita, Justin M.;Parker, Lauren E.;Wightman, R. Mark
通讯作者：
Wightman, R. Mark
影响因子：
3
作者：
KAWAGOE, KT;ZIMMERMAN, JB;WIGHTMAN, RM
通讯作者：
WIGHTMAN, RM
影响因子：
56.9
作者：
EWING, AG;BIGELOW, JC;WIGHTMAN, RM
通讯作者：
WIGHTMAN, RM
影响因子：
4.2
作者：
Heien, MLAV;Phillips, PEM;Wightman, RM
通讯作者：
Wightman, RM
影响因子：
7.4
作者：
Bath, BD;Michael, DJ;Wightman, RM
通讯作者：
Wightman, RM