Comparative Transcriptome Analysis Provides Novel Insight into Morphologic and Metabolic Changes in the Fat Body during Silkworm Metamorphosis.
Comparative Transcriptome Analysis Provides Novel Insight into Morphologic and Metabolic Changes in the Fat Body during Silkworm Metamorphosis.
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DOI：
10.3390/ijms19113525
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发表时间：
2018-11-09
影响因子：
5.6
通讯作者：
Zhao P
中科院分区：
文献类型：
--
作者：
Peng J;Li Z;Yang Y;Wang P;Zhou X;Zhao T;Guo M;Meng M;Zhang T;Qian W;Xia Q;Cheng D;Zhao P
脂肪体在昆虫体内能量的储存和利用以及生物合成和代谢活动中起着关键作用。从幼虫到蛹的变态过程中，脂肪体的形态和代谢过程发生了巨大的变化。然而，这些变化背后的遗传基础尚未完全了解。在这项研究中，作者使用RNA测序技术对家蚕变态期间的脂肪体进行了时程转录组分析。在不同发育时间点的脂肪体中共鉴定出5217个差异表达基因（DEG）。DEG参与脂质的合成和降解的高表达在第三天的最后一个龄期的幼虫和蛹前蛹蛹的过渡，分别。DEG参与蜕皮激素信号和骨形态发生蛋白（BMP）信号通路，调节器官发育表现出高表达水平的脂肪体重塑过程中，从预蛹到蛹。有趣的是，RNA干扰介导的decapentaplegic（Dpp）或蛋白60 A（Gbb）（两个参与BMP信号通路的DEG）的敲低抑制脂肪体解离，但促进脂质动员，表明BMP信号通路不仅是脂肪体重塑所需的，而且适度抑制脂质动员，以确保在蛹-成虫过渡期间适当的脂质供应。总之，比较转录组分析提供了新的见解，在家蚕变态过程中的脂肪体的形态和代谢变化。
The fat body plays key roles in energy storage and utilization as well as biosynthetic and metabolic activities in insects. During metamorphosis from larva to pupa, the fat body undergoes dramatic changes in morphology and metabolic processes. However, the genetic basis underlying these changes has not been completely understood. In this study, the authors performed a time-course transcriptome analysis of the fat body during silkworm metamorphosis using RNA-sequencing. A total of 5217 differentially expressed genes (DEGs) were identified in the fat body at different developmental time points. DEGs involved in lipid synthesis and degradation were highly expressed at the third day of the last larval instar and during the prepupal-pupal transition, respectively. DEGs involved in the ecdysone signaling and bone morphogenetic protein (BMP) signaling pathways that modulate organ development exhibited a high expression level during the fat body remodeling process from prepupa to pupa. Intriguingly, the RNA interference-mediated knockdown of either decapentaplegic (Dpp) or protein 60A (Gbb), two DEGs involved in the BMP signaling pathway, inhibited fat body dissociation but promoted lipid mobilization, suggesting that the BMP signaling pathway not only is required for fat body remodeling, but also moderately inhibits lipid mobilization to ensure an appropriate lipid supply during the pupal-adult transition. In conclusion, the comparative transcriptome analysis provides novel insight into morphologic and metabolic changes in the fat body during silkworm metamorphosis.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
5.2
作者：
Choe SS;Huh JY;Hwang IJ;Kim JI;Kim JB
通讯作者：
Kim JB
影响因子：
4.6
作者：
Cherbas, L;Hu, X;Cherbas, P
通讯作者：
Cherbas, P
影响因子：
3.7
作者：
Lindmo, Karine;Simonsen, Anne;Stenmark, Harald
通讯作者：
Stenmark, Harald
影响因子：
23.8
作者：
Arrese EL;Soulages JL
通讯作者：
Soulages JL