A Whole Virion Vaccine for COVID-19 Produced via a Novel Inactivation Method and Preliminary Demonstration of Efficacy in an Animal Challenge Model.
A Whole Virion Vaccine for COVID-19 Produced via a Novel Inactivation Method and Preliminary Demonstration of Efficacy in an Animal Challenge Model.
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DOI：
10.3390/vaccines9040340
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发表时间：
2021-04-01
期刊：
影响因子：
7.8
通讯作者：
Goodrich RP
中科院分区：
文献类型：
--
作者：
Ragan IK;Hartson LM;Dutt TS;Obregon-Henao A;Maison RM;Gordy P;Fox A;Karger BR;Cross ST;Kapuscinski ML;Cooper SK;Podell BK;Stenglein MD;Bowen RA;Henao-Tamayo M;Goodrich RP
COVID-19大流行引发了人们对快速开发和评估该疾病和其他新兴疾病候选疫苗的浓厚兴趣。为应对预防COVID-19传播的迫切需要，目前正在对几种制备候选疫苗的新方法进行临床评估。在许多情况下，这些方法依赖于疫苗生产和免疫刺激的新方法。我们报告了使用一种新的方法（SolaVAX）生产灭活疫苗候选人和测试的候选人在仓鼠动物模型中的能力，以防止感染后的挑战与SARS-CoV-2病毒。本工作中采用的研究包括评价接种后产生的中和抗体水平、产生的RBD和刺突蛋白的特异性抗体亚型水平、评价攻毒后病毒脱落、细胞组分的流式细胞术和单细胞测序数据以及攻毒后组织的组织病理学评价。该初步评价的结果提供了对作为接种拟议候选疫苗的结果而发生的免疫应答的深入了解，以及为促进Th 1型免疫应答而专门开发的佐剂制剂对疫苗效力的影响，以及在用活SARS-CoV-2攻击后对感染的保护。这些数据可能在开发有效的候选疫苗方面具有广泛的实用性。此外，该初步评估的结果表明，使用这种特定的光化学方法制备COVID-19的全病毒体疫苗可能在制备此类候选疫苗中具有潜在的实用性。
The COVID-19 pandemic has generated intense interest in the rapid development and evaluation of vaccine candidates for this disease and other emerging diseases. Several novel methods for preparing vaccine candidates are currently undergoing clinical evaluation in response to the urgent need to prevent the spread of COVID-19. In many cases, these methods rely on new approaches for vaccine production and immune stimulation. We report on the use of a novel method (SolaVAX) for production of an inactivated vaccine candidate and the testing of that candidate in a hamster animal model for its ability to prevent infection upon challenge with SARS-CoV-2 virus. The studies employed in this work included an evaluation of the levels of neutralizing antibody produced post-vaccination, levels of specific antibody sub-types to RBD and spike protein that were generated, evaluation of viral shedding post-challenge, flow cytometric and single cell sequencing data on cellular fractions and histopathological evaluation of tissues post-challenge. The results from this preliminary evaluation provide insight into the immunological responses occurring as a result of vaccination with the proposed vaccine candidate and the impact that adjuvant formulations, specifically developed to promote Th1 type immune responses, have on vaccine efficacy and protection against infection following challenge with live SARS-CoV-2. This data may have utility in the development of effective vaccine candidates broadly. Furthermore, the results of this preliminary evaluation suggest that preparation of a whole virion vaccine for COVID-19 using this specific photochemical method may have potential utility in the preparation of one such vaccine candidate.
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影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
DOI：
10.1099/jgv.0.001453
发表时间：
2020-09
期刊：
The Journal of general virology
影响因子：
--
作者：
Ogando NS;Dalebout TJ;Zevenhoven-Dobbe JC;Limpens RWAL;van der Meer Y;Caly L;Druce J;de Vries JJC;Kikkert M;Bárcena M;Sidorov I;Snijder EJ
通讯作者：
Snijder EJ
影响因子：
3.3
作者：
Martin, CB;Wilfong, E;Platz, M
通讯作者：
Platz, M
DOI：
10.1099/jgv.0.001567
发表时间：
2021-03
期刊：
The Journal of general virology
影响因子：
--
作者：
Monchatre-Leroy E;Lesellier S;Wasniewski M;Picard-Meyer E;Richomme C;Boué F;Lacôte S;Murri S;Pulido C;Vulin J;Salguero FJ;Gouilh MA;Servat A;Marianneau P
通讯作者：
Marianneau P
宿主病毒感染图谱揭示了重症 COVID-19 患者的特征
影响因子：
64.5
作者：
Bost, Pierre;Giladi, Amir;Amit, Ido
通讯作者：
Amit, Ido