In vitro susceptibility of filamentous fungal isolates from a corneal ulcer clinical trial.
In vitro susceptibility of filamentous fungal isolates from a corneal ulcer clinical trial.
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DOI：
10.1016/j.ajo.2013.10.004
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发表时间：
2014-02
影响因子：
4.2
通讯作者：
Lietman, Thomas M.
中科院分区：
文献类型：
--
作者：
Lalitha, Prajna;Sun, Catherine Q.;Prajna, N. Venkatesh;Karpagam, Rajarathinam;Geetha, Manoharan;O'Brien, Kieran S.;Cevallos, Vicky;McLeod, Stephen D.;Acharya, Nisha R.;Lietman, Thomas M.
描述真菌分离株对纳他霉素和伏立康唑的最低抑菌浓度（MIC），并将这些MIC与先前的眼部敏感性研究进行比较。使用随机临床试验分离株进行的实验室研究。肌层溃疡治疗试验I是一项随机、双盲、多中心试验，比较局部那他霉素和伏立康唑治疗真菌性角膜炎。根据临床和实验室标准协会的方法进行了对那他霉素和伏立康唑的敏感性试验。评估微生物与MIC之间的关系。进行了文献综述，将结果与先前的眼部易感性研究进行比较。在入组试验的323例患者中，221例（68%）的MIC可用。镰刀菌属（N=126）和曲霉属（N=52）是最常见的分离微生物。各属间纳他霉素和伏立康唑的MIC差异有统计学意义（P<0.001）。对于除弯孢属外的所有微生物，那他霉素的MIC中位数（MIC50）和第90百分位数（MIC90）等于或高于伏立康唑。与其他微生物相比，镰刀菌对伏立康唑和A.黄曲霉分离株对那他霉素的MIC最高。除伏立康唑对曲霉菌的MIC90高2倍，纳他霉素对曲霉菌的MIC90高2倍外，其他结果与文献报道相似。在我们的研究中，烟曲霉菌高4倍。在这项大规模的敏感性研究中，镰刀菌分离株对伏立康唑和A.与其它丝状真菌相比，黄曲霉分离物对那他霉素最不敏感。将来，如果及时进行，药敏试验可能有助于指导治疗。
To describe the minimum inhibitory concentration (MIC) of fungal isolates to natamycin and voriconazole, and to compare these MICs to previous ocular susceptibility studies. Experimental laboratory study using isolates from a randomized clinical trial. The Mycotic Ulcer Treatment Trial I was a randomized, double-masked, multicenter trial comparing topical natamycin and voriconazole for fungal keratitis treatment. Susceptibility testing to natamycin and voriconazole were performed according to Clinical and Laboratory Standards Institute methods. The relationship between organism and MIC was assessed. A literature review was performed to compare results to previous ocular susceptibility studies. Of the 323 patients enrolled in the trial, MICs were available for 221 (68%). Fusarium (N=126) and Aspergillus species (N=52) were the most commonly isolated organisms. MICs to natamycin and voriconazole were significantly different across all genera (P<0.001). The MIC median (MIC50) and 90th percentile (MIC90) for natamycin were equal to or higher than voriconazole for all organisms, except Curvularia species. Compared to other organisms, Fusarium species isolates had the highest MICs to voriconazole and A. flavus isolates had the highest MICs to natamycin. Our results were similar to previous reports except the voriconazole MIC90 against Aspergillus species was 2-fold higher and the natamycin MIC90 against A. fumigatus was 4-fold higher in our study. In this large susceptibility study, Fusarium isolates were least susceptible to voriconazole and A. flavus isolates were least susceptible to natamycin when compared to other filamentous fungi. In the future, susceptibility testing may help guide therapy if performed in a timely manner.
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DOI：
10.1097/icl.0b013e318235c5af
发表时间：
2012-09-01
影响因子：
2.3
作者：
Edelstein, Sean L.;Akduman, Levent;Hsu, Hugo Y.
通讯作者：
Hsu, Hugo Y.
影响因子：
4.2
作者：
JONES, BR
通讯作者：
JONES, BR
影响因子：
9.4
作者：
Lalitha, P.;Vijaykumar, R.;Fothergill, A. W.
通讯作者：
Fothergill, A. W.
影响因子：
2.8
作者：
ODAY, DM
通讯作者：
ODAY, DM
影响因子：
4.9
作者：
Homa, Monika;Shobana, Coimbatore S.;Galgoczy, Laszlo
通讯作者：
Galgoczy, Laszlo