Dynamic metabolic profiling of the marine microalga Chlamydomonas sp. JSC4 and enhancing its oil production by optimizing light intensity.
Dynamic metabolic profiling of the marine microalga Chlamydomonas sp. JSC4 and enhancing its oil production by optimizing light intensity.
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DOI：
10.1186/s13068-015-0226-y
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发表时间：
2015
影响因子：
6.3
通讯作者：
Kondo A
中科院分区：
文献类型：
--
作者：
Ho SH;Nakanishi A;Ye X;Chang JS;Chen CY;Hasunuma T;Kondo A
海洋微藻是生物柴油生产中最有前途的脂质来源之一，因为它们可以在不使用饮用水的情况下在非耕地上生长。海洋微藻还以高生长率有效地收集太阳能，将二氧化碳转化为储存在细胞中的脂质。光照强度和氮素供应对产油微藻的生长、油脂积累和脂肪酸组成均有显著影响。然而，很少有研究系统地研究了如何通过调节辐照强度来优化脂质生产率，并且可能导致微藻中脂质积累改善的代谢动力学尚未阐明。微藻中脂类合成的调控机制目前还知之甚少。此外，很少有研究评估利用海洋微藻作为石油生产者的潜力。在这项工作中，一个新分离的海洋微生物，衣原体属。JSC 4，被选为一个潜在的脂质生产商，和光生物反应器的操作对细胞生长和脂质生产的影响进行了研究。进一步探讨了光照强度和氮素耗竭胁迫对生长和油脂积累的综合影响，以期显著提高油脂产量和品质。获得的最佳脂质生产率和含量分别为312 mg L−1 d−1和43.1%/单位干细胞重量。这种脂质生产率是海洋微藻有史以来报道的最高水平。代谢中间体随时间的推移进行分析，以观察在脂质积累过程中的瞬态变化触发的组合应力。最后，代谢物周转率也进行了评估，使用体内13 C-标记技术，直接测量在与光照强度和氮缺乏相关的压力下脂质生物合成过程中的碳流。这项工作展示了培养和动态代谢分析技术的协同整合，以开发一种简单有效的策略来提高海洋微生物的石油产量。从这项研究中获得的知识可能是有用的，在评估海洋微藻生物柴油生产的可行性和了解微藻培养过程中的动态代谢谱和脂质生物合成之间的联系。本文的在线版本（doi：10.1186/s13068-015-0226-y）包含补充材料，可供授权用户使用。
Marine microalgae are among the most promising lipid sources for biodiesel production because they can be grown on nonarable land without the use of potable water. Marine microalgae also harvest solar energy efficiently with a high growth rate, converting CO2 into lipids stored in the cells. Both light intensity and nitrogen availability strongly affect the growth, lipid accumulation, and fatty acid composition of oleaginous microalgae. However, very few studies have systematically examined how to optimize lipid productivity by adjusting irradiance intensity, and the metabolic dynamics that may lead to improved lipid accumulation in microalgae have not been elucidated. Little is known about the mechanism of lipid synthesis regulation in microalgae. Moreover, few studies have assessed the potential of using marine microalgae as oil producers. In this work, a newly isolated marine microalga, Chlamydomonas sp. JSC4, was selected as a potential lipid producer, and the effect of photobioreactor operations on cell growth and lipid production was investigated. The combined effects of light intensity and nitrogen depletion stresses on growth and lipid accumulation were further explored in an effort to markedly improve lipid production and quality. The optimal lipid productivity and content attained were 312 mg L−1 d−1 and 43.1% per unit dry cell weight, respectively. This lipid productivity is the highest ever reported for marine microalgae. Metabolic intermediates were profiled over time to observe transient changes during lipid accumulation triggered by combined stresses. Finally, metabolite turnover was also assessed using an in vivo13C-labeling technique to directly measure the flow of carbon during lipid biosynthesis under stress associated with light intensity and nitrogen deficiency. This work demonstrates the synergistic integration of cultivation and dynamic metabolic profiling technologies to develop a simple and effective strategy for enhancing oil production in a marine microalga. The knowledge obtained from this study could be useful in assessing the feasibility of marine microalgae biodiesel production and for understanding the links between dynamic metabolic profiles and lipid biosynthesis during the course of microalgal cultivation. The online version of this article (doi:10.1186/s13068-015-0226-y) contains supplementary material, which is available to authorized users.
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影响因子：
11.4
作者：
Ho, Shih-Hsin;Li, Po-Jen;Chang, Jo-Shu
通讯作者：
Chang, Jo-Shu
DOI：
10.1007/s10295-009-0559-2
发表时间：
2009-06-01
影响因子：
3.4
作者：
Gouveia, Luisa;Marques, Ana Evangelista;Reis, Alberto
通讯作者：
Reis, Alberto
影响因子：
11.2
作者：
Jiang, Liling;Luo, Shengjun;Guo, Rongbo
通讯作者：
Guo, Rongbo
影响因子：
7.4
作者：
Bölling, C;Fiehn, O
通讯作者：
Fiehn, O
影响因子：
11.4
作者：
Cho, Sunja;Luong, Thanh Thao;Lee, Taeho
通讯作者：
Lee, Taeho