Trichosanthin-induced specific changes of cytoskeleton configuration were associated with the decreased expression level of actin and tubulin genes in apoptotic Hela cells.
Trichosanthin-induced specific changes of cytoskeleton configuration were associated with the decreased expression level of actin and tubulin genes in apoptotic Hela cells.
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天花粉素诱导的细胞骨架构型的特异性变化与凋亡 Hela 细胞中肌动蛋白和微管蛋白基因表达水平的降低有关。
DOI：
10.1016/j.lfs.2007.08.016
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发表时间：
2007-09
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
天花粉蛋白具有广泛的生物学和药理学活性，包括通过细胞凋亡途径发挥抗肿瘤作用。然而，TCS对Hela细胞凋亡过程中细胞骨架结构及肌动蛋白和微管蛋白基因表达的影响尚不清楚。在本研究中，凋亡的细胞骨架结构观察共聚焦免疫荧光显微镜，肌动蛋白和微管蛋白亚基的mRNA的绝对量通过定量实时PCR测定（QRT-PCR）。我们的研究结果表明，细胞凋亡的执行阶段是一个高度协调的细胞重组过程，解聚的微丝（MFs）积累在粗化的细胞质和凋亡小体，然后在质膜下形成环形微管（MT）结构。重要的是，细胞凋亡发生的抑制肌动蛋白和微管蛋白亚基基因的表达。在Hela细胞凋亡的后期，γ-actin mRNA的表达量先于β-actin mRNA的表达量迅速下降，α-和β-tubulin mRNA的表达量随之下调。这些结果表明，TCS诱导Hela细胞凋亡的过程伴随着细胞骨架结构的特异性改变，并在不同阶段显著降低了肌动蛋白和微管蛋白亚基基因的表达水平。
Trichosanthin (TCS) possesses a broad spectrum of biological and pharmacological activities, including anti-cancer activities through apoptosis pathway. However, little is known about the effects of TCS on the cytoskeleton configuration and expression of actin and tubulin genes in Hela cell apoptosis. In the present study, apoptotic cytoskeleton structures were observed by confocal immunofluorescence microscopy, absolute amounts of actin and tubulin subunit mRNAs were determined by quantitative real-time PCR assays (QRT-PCR). Our results showed that the execution phase of cell apoptosis was a highly coordinated process of cellular reorganization, depolymerized microfilaments (MFs) accumulated in the coarsened cytoplasm and apoptotic bodies, followed by the formation of a ring microtubule (MT) structure beneath the plasma membrane. Importantly, apoptosis occurred by a suppression of actin and tubulin subunit gene expression. In particular, a rapid decrease in the amounts of γ-actin mRNA preceded that of β-actin; α- and β-tubulin mRNAs were subsequently down-regulated in the later stage of Hela cell apoptosis. These results suggested that the execution of Hela cell apoptosis induced by TCS accompanied the specific changes of cytoskeleton configuration and, significantly, decreased the expression level of actin and tubulin subunit genes in different stages.
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影响因子：
64.8
作者：
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通讯作者：
Xuejun Zhang;Jiahuai Wang
影响因子：
5.3
作者：
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影响因子：
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影响因子：
11.2
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通讯作者：
Thomas G. Cotter;Seamus V. Lennon;Jacqueline M. Glynn;Douglas R. Green
影响因子：
4.1
作者：
COHEN, GM;SUN, XM;SKILLETER, DN
通讯作者：
SKILLETER, DN