Facially Amphipathic Glycopolymers Inhibit Ice Recrystallization.
Facially Amphipathic Glycopolymers Inhibit Ice Recrystallization.
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DOI：
10.1021/jacs.8b02066
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发表时间：
2018-05-02
影响因子：
15
通讯作者：
Gibson MI
中科院分区：
文献类型：
--
作者：
Graham B;Fayter AER;Houston JE;Evans RC;Gibson MI
来自极地鱼类的抗冻糖蛋白（AFGPs）是已知的最有效的冰重结晶（生长）抑制剂，并且需要合成模拟物用于低温应用，如细胞冷冻保存。在这里，我们介绍了模拟AFGPs三维结构的两亲性糖基共聚物。采用开环易位聚合法制备了具有分离的亲水和疏水表面的糖基共聚物，并用小角中子散射证实了其刚性构象。冰重结晶抑制（IRI）的活性降低时，亲水性氧代醚安装在聚糖的相对面，但显着的活性恢复通过将疏水性二甲基富烯残基。这种仿生策略表明，具有不同亲水性/疏水性的分离结构域是引入IRI活性的关键基序，这增加了我们对复杂的冰晶抑制过程的理解。
Antifreeze glycoproteins (AFGPs) from polar fish are the most potent ice recrystallization (growth) inhibitors known, and synthetic mimics are required for low-temperature applications such as cell cryopreservation. Here we introduce facially amphipathic glycopolymers that mimic the three-dimensional structure of AFGPs. Glycopolymers featuring segregated hydrophilic and hydrophobic faces were prepared by ring-opening metathesis polymerization, and their rigid conformation was confirmed by small-angle neutron scattering. Ice recrystallization inhibition (IRI) activity was reduced when a hydrophilic oxo-ether was installed on the glycan-opposing face, but significant activity was restored by incorporating a hydrophobic dimethylfulvene residue. This biomimetic strategy demonstrates that segregated domains of distinct hydrophilicity/hydrophobicity are a crucial motif to introduce IRI activity, which increases our understanding of the complex ice crystal inhibition processes.
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DOI：
10.1002/anie.201706703
发表时间：
2017-12-11
期刊：
Angewandte Chemie (International ed. in English)
影响因子：
--
作者：
Graham B;Bailey TL;Healey JRJ;Marcellini M;Deville S;Gibson MI
通讯作者：
Gibson MI
DOI：
10.1039/c5cc04647e
发表时间：
2015-08-21
期刊：
Chemical communications (Cambridge, England)
影响因子：
--
作者：
Mitchell DE;Cameron NR;Gibson MI
通讯作者：
Gibson MI
影响因子：
16.6
作者：
Biggs CI;Bailey TL;Ben Graham;Stubbs C;Fayter A;Gibson MI
通讯作者：
Gibson MI
影响因子：
15
作者：
Ilker, MF;Nüsslein, K;Coughlin, EB
通讯作者：
Coughlin, EB
影响因子：
4.6
作者：
Capicciotti CJ;Kurach JD;Turner TR;Mancini RS;Acker JP;Ben RN
通讯作者：
Ben RN