Interactions between FLORAL ORGAN NUMBER4 and floral homeotic genes in regulating rice flower development.
Interactions between FLORAL ORGAN NUMBER4 and floral homeotic genes in regulating rice flower development.
复制标题
花器官 NUMBER4 与花同源异型基因在调节水稻花发育中的相互作用
DOI：
10.1093/jxb/erw459
复制
发表时间：
2017-01-01
影响因子：
6.9
通讯作者：
Zhang D
中科院分区：
文献类型：
--
作者：
Xu W;Tao J;Chen M;Dreni L;Luo Z;Hu Y;Liang W;Zhang D
水稻花的发育决定着产量。本研究揭示了水稻分生组织维持基因FON 4与6个花同源异型基因在花发育中的遗传互作关系。花分生组织（FM）在花发育的早期阶段是自我维持的，但当花器官产生一定数量时，它就终止了。花器官编号4（FON 4;也称为FON 2）基因是拟南芥CLAVATA 3（CLV 3）的直系同源基因，是调节水稻中FM大小和确定性所必需的。然而，它与花同源异型基因的相互作用仍然未知。本文报道了FON 4与花同源异型基因OsMADS 15（A类基因）、OsMADS 16（B类基因）、OsMADS 3和OsMADS 58（C类基因）、OsMADS 13（D类基因）和OsMADS 1（E类基因）在花发育过程中的遗传互作。我们在具有OsMADS 15突变等位基因dep（退化内稃）的fon 4双突变体中观察到加性表型。在fon 4 spw 1中，对第2轮器官数的影响增强。FON 4与osmads 3、osmads 58、osmads 13和osmads 1的双突变体组合分别显示出FM确定性和同一性的增强缺陷，表明FON 4和这些基因协同控制FM活性。此外，在fon 4突变体中，除OsMADS 13基因外，其他基因的表达模式均无明显变化。这项工作揭示了分生组织维持基因FON 4如何与C，D和E花同源异型基因在指定FM活动在单子叶水稻遗传相互作用。
Rice flower development determines grain yield. Here we reveal the genetic interactions between the rice meristem maintenance gene FON4 and six floral homeotic genes in flower development. The floral meristem (FM) is self-maintaining at the early stages of flower development, but it is terminated when a fixed number of floral organs are produced. The FLORAL ORGAN NUMBER4 (FON4; also known as FON2) gene, an ortholog of Arabidopsis CLAVATA3 (CLV3), is required for regulating FM size and determinacy in rice. However, its interactions with floral homeotic genes remain unknown. Here, we report the genetic interactions between FON4 and floral homeotic genes OsMADS15 (an A-class gene), OsMADS16 (also called SUPERWOMAN1, SPW1, a B-class gene), OsMADS3 and OsMADS58 (C-class genes), OsMADS13 (a D-class gene), and OsMADS1 (an E-class gene) during flower development. We observed an additive phenotype in the fon4 double mutant with the OsMADS15 mutant allele dep (degenerative palea). The effect on the organ number of whorl 2 was enhanced in fon4 spw1. Double mutant combinations of fon4 with osmads3, osmads58, osmads13, and osmads1 displayed enhanced defects in FM determinacy and identity, respectively, indicating that FON4 and these genes synergistically control FM activity. In addition, the expression patterns of all the genes besides OsMADS13 had no obvious change in the fon4 mutant. This work reveals how the meristem maintenance gene FON4 genetically interacts with C, D, and E floral homeotic genes in specifying FM activity in monocot rice.
登录
查看更多内容
影响因子：
64.5
作者：
DREWS, GN;BOWMAN, JL;MEYEROWITZ, EM
通讯作者：
MEYEROWITZ, EM
影响因子：
11.6
作者：
Dreni, Ludovico;Pilatone, Alessandro;Kater, Martin M.
通讯作者：
Kater, Martin M.
影响因子：
7.4
作者：
Bleckmann, Andrea;Weidtkamp-Peters, Stefanie;Simon, Ruediger
通讯作者：
Simon, Ruediger
影响因子：
64.8
作者：
Bommert, Peter;Il Je, Byoung;Jackson, David
通讯作者：
Jackson, David
影响因子：
5.1
作者：
Agrawal, G;Abe, K;Hirochika, H
通讯作者：
Hirochika, H