Diffusion tensor imaging of ex vivo cervical spinal cord specimens: the immediate and long-term effects of fixation on diffusivity.
Diffusion tensor imaging of ex vivo cervical spinal cord specimens: the immediate and long-term effects of fixation on diffusivity.
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DOI：
10.1002/ar.20823
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发表时间：
2009-02
影响因子：
2
通讯作者：
Jeong, E. -K.
中科院分区：
文献类型：
--
作者：
Kim, T. H.;Zollinger, L.;Shi, X. F.;Rose, J.;Jeong, E. -K.
扩散张量成像（Diffusion Tensor imaging， DTI）是一种新兴的无创组织显微结构评估方法，但它极易受到活体运动伪影的影响。为了改进需要固定组织标本的DTI技术，需要在固定组织上进行离体实验。研究固定对人类和小鼠组织的影响已经做了一些努力，结果各不相同。采用三维多次弥散加权成像技术（ms-DWEPI）对组织固定前后的4条人颈脊髓和3段猪体内颈脊髓标本进行成像。固定导致纵向扩散率显著降低，而相对各向异性（RA）和径向扩散率未受影响。此外，一旦充分保存，固定组织的扩散系数参数随时间保持不变。固定对组织标本的扩散率有重要影响。这些发现对利用DTI技术测定组织微观结构和功能具有重要意义。
Diffusion Tensor imaging (DTI) is an emerging noninvasive method for evaluating tissue microstructure, but is highly susceptible to in vivo motion artifact. Ex vivo experiments on fixed tissues are needed to improve DTI techniques, which require fixed tissue specimens. Several efforts have been made to study the effect of fixation on both human and mouse tissue, with varying results. Four human cervical cords and three segments of pig vivo cervical spinal cord specimens were imaged both before and after tissue fixation using 3D multi-shot diffusion weighted imaging (ms-DWEPI). Fixation caused a significant decrease in the longitudinal diffusivity while the relative anisotropy (RA), and radial diffusivity remained unaffected. Additionally, once adequately preserved the diffusivity parameters of fixed tissue remain constant over time. Fixation has important effects on the diffusivity of tissue specimens. These findings have important implications for the determination of tissue microstructure and function using DTI technologies.
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