State-of-the-art CRISPR for in vivo and cell-based studies in Drosophila.
State-of-the-art CRISPR for in vivo and cell-based studies in Drosophila.
复制标题
果蝇中的体内和基于细胞的研究的最先进的CRISPR。
DOI：
10.1016/j.tig.2021.11.006
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发表时间：
2022-05
影响因子：
11.4
通讯作者：
Perrimon, Norbert
中科院分区：
文献类型：
--
作者：
Zirin, Jonathan;Bosch, Justin;Viswanatha, Raghuvir;Mohr, Stephanie E.;Perrimon, Norbert
一百多年来，果蝇（Drosophila melanogaster）一直是生物学和生物医学研究的强大模式生物，因为它在遗传和生理上与人类有许多相似之处，而且可以利用复杂的技术来操纵它的基因组和基因。果蝇研究界迅速采用了CRISPR技术，自首次在果蝇中发表CRISPR以来的8年里，已经探索和创新了诱变、精确基因组工程等方法。此外，果蝇的短寿命和易于遗传使其成为快速发展的CRISPR-Cas工具在体内应用和改进的理想试验场。在这里，我们回顾了CRISPR试剂递送方面的创新，提高了切割和HDR效率，以及标准的基于cas9的方法的替代方案。虽然重点主要集中在体内系统，但我们也描述了果蝇培养细胞的作用，既是评估新CRISPR技术过程中不可或缺的第一步，也是全基因组CRISPR汇集筛选的平台。
For more than a hundred years, the fruit fly, Drosophila melanogaster, has served as a powerful model organism for biological and biomedical research due to its many genetic and physiological similarities to humans and availability of sophisticated technologies used to manipulate its genome and genes. The Drosophila research community quickly adopted CRISPR technologies and, in the 8 years since the first CRISPR publications in flies, has explored and innovated methods for mutagenesis, precise genome engineering, and beyond. Moreover, the short lifespan and ease of genetics have made Drosophila an ideal testing ground for in vivo applications and refinements of the rapidly evolving set of CRISPR-Cas tools. Here, we review innovations in delivery of CRISPR reagents, increased efficiency of cutting and HDR, and alternatives to standard Cas9-based approaches. While the focus is primarily on in vivo systems, we also describe the role of Drosophila cultured cells as both an indispensable first step in the process of assessing new CRISPR technologies and a platform for genome-wide CRISPR pooled screens.
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影响因子：
48
作者：
Chavez A;Tuttle M;Pruitt BW;Ewen-Campen B;Chari R;Ter-Ovanesyan D;Haque SJ;Cecchi RJ;Kowal EJK;Buchthal J;Housden BE;Perrimon N;Collins JJ;Church G
通讯作者：
Church G
影响因子：
3.8
作者：
Cristea, Sandra;Freyvert, Yevgeniy;Cost, Gregory J.
通讯作者：
Cost, Gregory J.
影响因子：
3.8
作者：
Frit, Philippe;Barboule, Nadia;Calsou, Patrick
通讯作者：
Calsou, Patrick
影响因子：
25
作者：
Garcia-Marques, Jorge;Espinosa-Medina, Isabel;Lee, Tzumin
通讯作者：
Lee, Tzumin
DOI：
10.1073/pnas.2021996118
发表时间：
2021-01-05
影响因子：
11.1
作者：
Bosch, Justin A.;Birchak, Gabriel;Perrimon, Norbert
通讯作者：
Perrimon, Norbert