Interaction between mismatch repair and genetic recombination in Saccharomyces cerevisiae.
Interaction between mismatch repair and genetic recombination in Saccharomyces cerevisiae.
复制标题
酿酒酵母错配修复和基因重组之间的相互作用。
DOI：
10.1093/genetics/137.1.19
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发表时间：
1994
期刊：
影响因子：
3.3
通讯作者：
Kolodner,RD
中科院分区：
文献类型：
--
作者：
Alani,E;Reenan,RA;Kolodner,RD
酵母酿酒酵母编码一组基因，这些基因显示出与大肠杆菌中错配修复所需的MutS和MutL蛋白的强烈氨基酸序列相似性。我们研究了分别为mutS和mutL的酵母同源物MSH 2和PMS 1在修复减数分裂重组期间形成的碱基对错配中的作用。通过使用特异性标记的HIS 4和ARG 4等位基因，我们发现msh 2突变体在所有碱基对错配以及1-，2-和4-bp插入/缺失错配的修复中显示出严重的缺陷。msh 2和pms 1的表型是不可区分的，这表明野生型基因产物在相同的修复途径中起作用。野生型和msh 2突变体中基因转换事件的比较表明错配修复在遗传重组中起着重要作用。(1)在5个不同位点的四分体分析显示，在msh 2突变体中，大多数异常分离子显示扇形表型，与未能修复异源双链体形成过程中产生的错配一致。在野生型中，碱基对错配几乎完全被修复为转化而不是恢复。(2)在msh 2菌株中，10-19%的异常四分体是Ab 4：4。(3)在msh 2突变体中，HIS 4和ARG 4的极性梯度几乎被消除。在这些基因的3'端的基因转换频率增加，并且接近在5'端观察到的频率。(4)共转化研究与错配修复作用于调节异源双链DNA段长度一致。我们赞成一个模型，提出重组事件发生通过异源双链中间体的形成和解决，错配修复蛋白特异性地与重组酶相互作用，以调节对称异源双链DNA的长度。
The yeast Saccharomyces cerevisiae encodes a set of genes that show strong amino acid sequence similarity to MutS and MutL, proteins required for mismatch repair in Escherichia coli. We examined the role of MSH2 and PMS1, yeast homologs of mutS and mutL, respectively, in the repair of base pair mismatches formed during meiotic recombination. By using specifically marked HIS4 and ARG4 alleles, we showed that msh2 mutants displayed a severe defect in the repair of all base pair mismatches as well as 1-, 2- and 4-bp insertion/deletion mispairs. The msh2 and pms1 phenotypes were indistinguishable, suggesting that the wild-type gene products act in the same repair pathway. A comparison of gene conversion events in wild-type and msh2 mutants indicated that mismatch repair plays an important role in genetic recombination. (1) Tetrad analysis at five different loci revealed that, in msh2 mutants, the majority of aberrant segregants displayed a sectored phenotype, consistent with a failure to repair mismatches created during heteroduplex formation. In wild type, base pair mismatches were almost exclusively repaired toward conversion rather than restoration. (2) In msh2 strains 10-19% of the aberrant tetrads were Ab4:4. (3) Polarity gradients at HIS4 and ARG4 were nearly abolished in msh2 mutants. The frequency of gene conversion at the 3' end of these genes was increased and was nearly the frequency observed at the 5' end. (4) Co-conversion studies were consistent with mismatch repair acting to regulate heteroduplex DNA tract length. We favor a model proposing that recombination events occur through the formation and resolution of heteroduplex intermediates and that mismatch repair proteins specifically interact with recombination enzymes to regulate the length of symmetric heteroduplex DNA.
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影响因子：
3.3
作者：
Stapleton,A;Petes,TD
通讯作者：
Petes,TD
影响因子：
3.3
作者：
M. Lichten;R. Borts;James E. Haber
通讯作者：
M. Lichten;R. Borts;James E. Haber
酵母转化过程中相似但不相同的 DNA 序列之间的重组发生在短的同一性范围内
影响因子：
64.5
作者：
C. Mézard;D. Pompon;A. Nicolas
通讯作者：
A. Nicolas
影响因子：
3.3
作者：
M. S. Williamson;J. Game;S. Fogel
通讯作者：
M. S. Williamson;J. Game;S. Fogel
由重复酵母基因之间的高频减数分裂重组产生的染色体易位。
影响因子：
3.3
作者：
Jinks-Robertson,S;Petes,TD
通讯作者：
Petes,TD