Pathogenesis and antigenic characterization of a new East European subtype 3 porcine reproductive and respiratory syndrome virus isolate.
Pathogenesis and antigenic characterization of a new East European subtype 3 porcine reproductive and respiratory syndrome virus isolate.
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DOI：
10.1186/1746-6148-6-30
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发表时间：
2010-06-04
影响因子：
2.6
通讯作者：
Nauwynck HJ
中科院分区：
文献类型：
--
作者：
Karniychuk UU;Geldhof M;Vanhee M;Van Doorsselaere J;Saveleva TA;Nauwynck HJ
猪繁殖与呼吸综合征病毒(PRRSV)分为欧洲和北美两种基因型。东欧PRRSV分离株已被发现属于欧洲基因型，但形成不同的亚型。在本研究中，PRRSV是从白俄罗斯一个患有生殖和呼吸衰竭的农场分离出来的，命名为“Lena”。分析表明，Lena是一种新的东欧3型PRRSV分离株。本研究的主要目的是研究PRRSV(LENA)的致病机理和抗原性。在接种新近的欧洲1型PRRSV分离株(比利时A)的动物和接种PRRSV的动物(Lena)之间，观察到明显的临床和病毒学差异。在接种PRRSV(比利时A)的6头猪中，有3头在接种后3、4和5天出现厌食和低烧。在2~28dpi之间接种PRRSV(Lena)的大多数猪都有明显的高热、食欲减退和抑郁症状。在实验中，十分之四的猪都死了。从一只死亡的动物的肺中分离出化脓性隐秘杆菌，从一只被安乐死的动物的肺中分离到猪链球菌。在感染PRRSV(比利时A)和PRRSV(Lena)的猪的血清中，病毒滴度在7、10、14和21dpi时差异有统计学意义(p<；0.05)。PRRSV(LENA)的血清最高滴度为104.8~106.1 TCID50/ml，而PRRSV(比利时A)的最高TCID50/ml为103.1~104.8 TCID50/ml。对PRRSV(Lena)的复制进行了深入研究。病毒滴度在3~14dpi的鼻分泌物中为102.5 TCID50/100 mg~105.6 TCID50/100 mg，在3~21dpi的扁桃体刮片中为102.8~104.6 TCID50/100 mg。病毒滴度在肺(102.3~107.7 TCID50/g组织)、扁桃体(102.0~106.2 TCID50/g组织)和腹股沟淋巴结(102.2~106.6 TCID50/g组织)分别高达35、28和35dpi。为了检测东欧3型毒株Lena、欧洲1型毒株Lelystad和北美毒株US5之间的抗原异质性，用同源和异种病毒抗原对一组单特异性多克隆抗血清进行了免疫过氧化物酶单层分析(IPMA)。在所有采样时间点，PRRSV(Lena)抗血清的异源抗体滴度均显著低于同源抗体滴度(p=0.01~0.03)。对于PRRSV(Lelystad)和PRRSV(US5)抗血清，异源抗体滴度分别在14和21dpi(p=0.01-0.03)和10和14dpi(p=0.04)显著低于同源抗体滴度(p=0.04)。Lena是一种高致病性的东欧3型PRRSV，在基因和抗原水平上都不同于欧洲1型Lelystad和北美的US5株。
Porcine reproductive and respiratory syndrome virus (PRRSV) is divided into a European and North American genotype. East European PRRSV isolates have been found to be of the European genotype, but form different subtypes. In the present study, PRRSV was isolated from a Belarusian farm with reproductive and respiratory failure and designated "Lena". Analyses revealed that Lena is a new East European subtype 3 PRRSV isolate. The main purpose of this investigation was to study the pathogenesis and antigenic characteristics of PRRSV (Lena). Obvious clinical and virological differences were observed between the animals inoculated with a recent European subtype 1 PRRSV isolate (Belgium A) and animals inoculated with PRRSV (Lena). Three out of six pigs inoculated with PRRSV (Belgium A) had anorexia and low fever at 3, 4 and 5 days post-inoculation (dpi). High fever, anorexia and depression were prominent signs in most pigs inoculated with PRRSV (Lena) between 2 and 28 dpi. Four pigs out of ten died during the experiment. Arcanobacterium pyogenes was isolated from lungs of one animal that died, and Streptococcus suis was isolated from lungs of one animal that was euthanized. The difference in viral titres in sera from PRRSV (Belgium A) and PRRSV (Lena)-infected pigs was statistically significant (p < 0.05) at 7, 10, 14 and 21 dpi. The highest viral titres in sera ranged from 104.8 to 106.1 TCID50/ml for PRRSV (Lena) whereas they ranged from 103.1 to 104.8 TCID50/ml for PRRSV (Belgium A). The replication of PRRSV (Lena) was further studied in depth. Viral titres ranged from 102.5 TCID50/100 mg to 105.6 TCID50/100 mg in nasal secretions between 3 and 14 dpi and from 102.8 TCID50/100 mg to 104.6 TCID50/100 mg in tonsillar scrapings between 3 and 21 dpi. High viral titres were detected in lungs (102.3-107.7 TCID50/g tissue), tonsils (102.0-106.2 TCID50/g tissue) and inguinal lymph nodes (102.2-106.6 TCID50/g tissue) until 35, 28 and 35 dpi, respectively. To examine the antigenic heterogeneity between the East European subtype 3 isolate Lena, the European subtype 1 strain Lelystad and the North American strain US5, sets of monospecific polyclonal antisera were tested in immunoperoxidase monolayer assays (IPMAs) with homologous and heterologous viral antigens. Heterologous antibody titres were significantly lower than homologous titres (p = 0.01-0.03) for antisera against PRRSV (Lena) at all sampling time points. For antisera against PRRSV (Lelystad) and PRRSV (US5), heterologous antibody titres were significantly lower than homologous titres at 14 and 21 dpi (p = 0.01-0.03) and at 10 and 14 dpi (p = 0.04), respectively. Lena is a highly pathogenic East European subtype 3 PRRSV, which differs from European subtype 1 Lelystad and North American US5 strains at both the genetic and antigenic level.
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影响因子：
1.8
作者：
Johnson, W;Roof, M;Murtaugh, MP
通讯作者：
Murtaugh, MP
猪呼吸与繁殖综合征病毒变种，越南和中国，2007 年。
影响因子：
11.8
作者：
Feng Y;Zhao T;Nguyen T;Inui K;Ma Y;Nguyen TH;Nguyen VC;Liu D;Bui QA;To LT;Wang C;Tian K;Gao GF
通讯作者：
Gao GF
影响因子：
3.7
作者：
Truong HM;Lu Z;Kutish GF;Galeota J;Osorio FA;Pattnaik AK
通讯作者：
Pattnaik AK
影响因子：
2.4
作者：
Thanawongnuwech, R;Halbur, PG;Royer, RL
通讯作者：
Royer, RL
影响因子：
3.8
作者：
Kapur, V;Elam, MR;Murtaugh, MP
通讯作者：
Murtaugh, MP