Rapid 3D bioprinting of decellularized extracellular matrix with regionally varied mechanical properties and biomimetic microarchitecture.
Rapid 3D bioprinting of decellularized extracellular matrix with regionally varied mechanical properties and biomimetic microarchitecture.
复制标题
DOI：
10.1016/j.biomaterials.2018.09.026
复制
发表时间：
2018-12
期刊：
影响因子：
14
通讯作者：
Chen S
中科院分区：
文献类型：
--
作者：
Ma X;Yu C;Wang P;Xu W;Wan X;Lai CSE;Liu J;Koroleva-Maharajh A;Chen S
肝细胞癌（HCC）是第五大常见恶性肿瘤，主要发生于肝硬化，肝内有纤维带分隔的硬结节。能够提供具有复杂天然组成和仿生结构的3D仿生力学环境的支架对于开发更好的预测性组织模型是必要的。在这里，我们开发了可光交联的肝脏脱细胞细胞外基质（dECM）和快速的基于光的3D生物打印过程，以使肝脏dECM具有可定制的机械特性，作为HCC进展研究的平台。3D生物打印的肝脏dECM支架能够稳定地重现肝脏组织的临床相关机械特性。与健康对照相比，当包封在具有抗肿瘤刚度的dECM支架中时，HepG2细胞表现出生长减少沿着侵袭标志物的上调。此外，具有组织规模组织和不同区域硬度的工程化癌症组织平台使得能够可视化HepG2基质从具有抗硬化硬度的结节侵入。这项工作证明了在复杂ECM生物材料的快速3D图案化方面的重大进展，该材料具有仿生结构和可调的机械性能，用于体外疾病建模。我们提出了一种快速的基于光的3D生物打印过程，以使肝脏dECM具有可定制的机械特性，作为HCC进展研究的平台。
Hepatocellular carcinoma (HCC), as the fifth most common malignant cancer, develops and progresses mostly in a cirrhotic liver where stiff nodules are separated by fibrous bands. Scaffolds that can provide a 3D cirrhotic mechanical environment with complex native composition and biomimetic architecture are necessary for the development of better predictive tissue models. Here, we developed photocrosslinkable liver decellularized extracellular matrix (dECM) and a rapid light-based 3D bioprinting process to pattern liver dECM with tailorable mechanical properties to serve as a platform for HCC progression study. 3D bioprinted liver dECM scaffolds were able to stably recapitulate the clinically relevant mechanical properties of cirrhotic liver tissue. When encapsulated in dECM scaffolds with cirrhotic stiffness, HepG2 cells demonstrated reduced growth along with an upregulation of invasion markers compared to healthy controls. Moreover, an engineered cancer tissue platform possessing tissue-scale organization and distinct regional stiffness enabled the visualization of HepG2 stromal invasion from the nodule with cirrhotic stiffness. This work demonstrates a significant advancement in rapid 3D patterning of complex ECM biomaterials with biomimetic architecture and tunable mechanical properties for in vitro disease modeling. We present a rapid light-based 3D bioprinting process to pattern liver dECM with tailorable mechanical properties to serve as a platform for HCC progression study.
登录
查看更多内容
影响因子：
16.6
作者：
Pati F;Jang J;Ha DH;Won Kim S;Rhie JW;Shim JH;Kim DH;Cho DW
通讯作者：
Cho DW
影响因子：
6.7
作者：
Kuyvenhoven, JP;van Hoek, B;Verspaget, HW
通讯作者：
Verspaget, HW
影响因子：
4.1
作者：
Park, Kyung-Mee;Hussein, Kamal Hany;Woo, Heung-Myong
通讯作者：
Woo, Heung-Myong
影响因子：
6.2
作者：
Lee, Jung Seung;Shin, Jisoo;Cho, Seung-Woo
通讯作者：
Cho, Seung-Woo
影响因子：
6.2
作者：
Lee, Hyungseok;Han, Wonil;Cho, Dong-Woo
通讯作者：
Cho, Dong-Woo