Microalgae as bioreactors for bioplastic production.
Microalgae as bioreactors for bioplastic production.
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DOI：
10.1186/1475-2859-10-81
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发表时间：
2011-10-17
影响因子：
6.4
通讯作者：
Maier UG
中科院分区：
文献类型：
--
作者：
Hempel F;Bozarth AS;Lindenkamp N;Klingl A;Zauner S;Linne U;Steinbüchel A;Maier UG
聚-3-羟基丁酸酯（PHB）是一种具有热塑性的聚酯，它是天然存在的，由真养罗尔斯通氏菌H16和巨大芽孢杆菌等细菌产生。与目前使用的塑料和大多数合成聚合物相比，PHB是可生物降解的，其生产不依赖于化石资源，使这种生物塑料对各种工业应用感兴趣。在这项研究中，我们报告了引入细菌的R。eutropha H16转化为硅藻三角褐指藻，从而首次证明在微藻系统中生产PHB是可行的。细菌酶的表达足以导致藻干重的10.6%的PHB水平。如光学和电子显微镜所示，生物塑料在细胞的胞质溶胶中以颗粒状结构积累。我们的研究证明了微藻如硅藻三角褐指藻作为太阳能表达工厂的巨大潜力，并揭示了与基于植物的生产系统相比的巨大优势。
Poly-3-hydroxybutyrate (PHB) is a polyester with thermoplastic properties that is naturally occurring and produced by such bacteria as Ralstonia eutropha H16 and Bacillus megaterium. In contrast to currently utilized plastics and most synthetic polymers, PHB is biodegradable, and its production is not dependent on fossil resources making this bioplastic interesting for various industrial applications. In this study, we report on introducing the bacterial PHB pathway of R. eutropha H16 into the diatom Phaeodactylum tricornutum, thereby demonstrating for the first time that PHB production is feasible in a microalgal system. Expression of the bacterial enzymes was sufficient to result in PHB levels of up to 10.6% of algal dry weight. The bioplastic accumulated in granule-like structures in the cytosol of the cells, as shown by light and electron microscopy. Our studies demonstrate the great potential of microalgae like the diatom P. tricornutum to serve as solar-powered expression factories and reveal great advantages compared to plant based production systems.
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影响因子：
4.4
作者：
BRANDL, H;GROSS, RA;FULLER, RC
通讯作者：
FULLER, RC
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Daniell, Henry;Singh, Nameirakpam D.;Mason, Hugh;Streatfield, Stephen J.
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Streatfield, Stephen J.
影响因子：
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WARD, OP
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7.4
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