Identification of a G2-like porcine rotavirus bearing a novel VP4 type, P[32].
Identification of a G2-like porcine rotavirus bearing a novel VP4 type, P[32].
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DOI：
10.1051/vetres/2010045
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发表时间：
2010-09
影响因子：
4.4
通讯作者：
O'Shea H
中科院分区：
文献类型：
--
作者：
Collins PJ;Martella V;Buonavoglia C;O'Shea H
在2007年对南爱尔兰猪群进行流行病学调查期间，从一头4-5周无症状仔猪中分离出猪A组轮状病毒（GARV） 61/07/Ire毒株。测定了61/07/Ire菌株VP4全长蛋白的核苷酸（nt）和氨基酸（aa）序列。基于整个VP4开放阅读框（nt），菌株61/07/Ire与已确定的31种P基因型的代表具有≤76.5%的一致性，远低于轮状病毒分类工作组（RCWG）确定的新P基因型的百分比一致性临界值（80%）。菌株61/07/Ire对不同P基因型代表的同源序列具有57.1% ~ 83.6%的低同源性。VP4的VP8*胰蛋白酶裂解片段（包含VP4高变区）aa同源性较低，为36.9% ~ 75.3%。对GARV 61/07/Ire菌株VP7、VP6和NSP4基因的序列分析表明，该菌株具有g2样VP7、E9样NSP4和I5样VP6基因型。综上所述，这些结果表明GARV 61/07/Ire毒株可被认为是新的VP4基因型P[32]的原型，并为a组轮状病毒的巨大异质性提供了进一步的证据。
A porcine group A rotavirus (GARV) strain, 61/07/Ire, was isolated from a 4–5 week asymptomatic piglet, during an epidemiological survey of porcine herds in Southern Ireland, in 2007. The nucleotide (nt) and amino acid (aa) sequence of the full-length VP4 protein of the PoRV strain 61/07/Ire was determined. Based on the entire VP4 open reading frame (nt), strain 61/07/Ire displayed ≤ 76.5% identity to representatives of the established 31 P-types, a value far lower than the percentage identity cutoff value (80%) established by the Rotavirus Classification Working Group (RCWG) to define a novel P genotype. Strain 61/07/Ire revealed low aa identity, ranging from 57.1% to 83.6%, to the cognate sequences of representatives of the various P genotypes. The aa identity was lower in the VP8* trypsin-cleavage fragment of the VP4, which encompasses the VP4 hypervariable region, ranging from 36.9% to 75.3%. Sequence analyses of the VP7, VP6, and NSP4 genes revealed that the GARV strain 61/07/Ire possessed a G2-like VP7, an E9 NSP4 genotype and an I5 VP6 genotype. Altogether, these results indicate that the GARV strain 61/07/Ire should be considered as a prototype of a new VP4 genotype, P[32], and provide further evidence for the vast heterogeneity of group A rotaviruses.
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影响因子：
3.7
作者：
Khamrin, Pattara;Maneekarn, Niwat;Ushijima, Hiroshi
通讯作者：
Ushijima, Hiroshi
影响因子：
9.4
作者：
GOUVEA, V;GLASS, RI;FANG, ZY
通讯作者：
FANG, ZY
影响因子：
9.4
作者：
Collins, P. J.;Martella, V.;O'Shea, H.
通讯作者：
O'Shea, H.
DOI：
10.1073/pnas.83.10.3465
发表时间：
1986-05-01
影响因子：
11.1
作者：
DYALLSMITH, ML;LAZDINS, I;HOLMES, IH
通讯作者：
HOLMES, IH
影响因子：
5.8
作者：
Kumar, S;Tamura, K;Nei, M
通讯作者：
Nei, M