Stress-induced tRNA-derived RNAs: a novel class of small RNAs in the primitive eukaryote Giardia lamblia.
Stress-induced tRNA-derived RNAs: a novel class of small RNAs in the primitive eukaryote Giardia lamblia.
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应激诱导的 tRNA 衍生 RNA：原始真核生物兰氏贾第鞭毛虫中的一类新型小 RNA。
DOI：
10.1093/nar/gkn596
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发表时间：
2008-11
影响因子：
14.9
通讯作者：
Qu LH
中科院分区：
文献类型：
--
作者：
Li Y;Luo J;Zhou H;Liao JY;Ma LM;Chen YQ;Qu LH
贾第鞭毛虫是一种早期分化和进化成功的原生动物，因为它可以从营养滋养体进入休眠的囊肿阶段。在休眠阶段，其代谢率急剧下降。然而，迄今为止，参与启动和维持这一过程的调节分子尚未得到充分的研究。在这项研究中，我们发现了一类丰富的小rna，称为sitRNAs，其长度约为46个核苷酸，并在兰氏螺旋体培养物中积累。值得注意的是，它们来自完全成熟的trna的3 ‘部分，通过反密码子左臂的切割，3 ’端CCA三联体仍然连接。在分化过程中，只有有限部分的成熟tRNA被切割，但这种切割几乎发生在整个tRNA家族中。sirna早在胞制起始后3小时就开始积累，并在整个过程中保持相对稳定的水平，在24小时左右达到表达高峰。我们的研究进一步表明，其他几种应激因素也可以诱导sitRNAs，在血清剥夺的情况下，tRNA和sitRNAs都能迅速降解，tRNA的积累减少一半。我们的研究结果可能为应激兰氏螺旋体调控基因表达的新机制提供新的见解。
Giardia lamblia is an early diverging and evolutionarily successful protozoan as it can enter into a dormant cyst stage from a vegetative trophozoite. During dormant stage, its metabolic rate decreases dramatically. However, to date, the regulatory molecules participating in the initiation and maintenance of this process have not been fully investigated. In this study, we have identified a class of abundant small RNAs named sitRNAs, which are ∼46 nucleotides in length and accumulate in G. lamblia encysting cultures. Remarkably, they are derived from the 3′ portion of fully matured tRNAs by cleavage of the anticodon left arm, with the 3′ terminal CCA triplex still connected. During differentiation, only a limited portion of mature tRNAs is cleaved, but this cleavage occurs almost in the entire tRNA family. sitRNAs begin to accumulate as early as 3 h after initiation of encystation and are maintained at a relatively stable level during the whole process, exhibiting an expression peak at around 24 hr. Our studies further show that sitRNAs can be induced by several other stress factors, and in the case of serum deprivation, both tRNAs and sitRNAs degrade rapidly, with the accumulation of tRNA being halved. Our results may provide new insight into a novel mechanism for stressed G. lamblia to regulate gene expression globally.
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影响因子：
14.9
作者：
Haiser HJ;Karginov FV;Hannon GJ;Elliot MA
通讯作者：
Elliot MA
影响因子：
4.5
作者：
Nelson, Audrey R.;Henkin, Tina M.;Agris, Paul F.
通讯作者：
Agris, Paul F.
DOI：
10.1073/pnas.0503836102
发表时间：
2005-08-09
影响因子：
11.1
作者：
Shaheen, HH;Hopper, AK
通讯作者：
Hopper, AK
影响因子：
10.7
作者：
Morrison, HG;Roger, AJ;Sogin, ML
通讯作者：
Sogin, ML
影响因子：
56.9
作者：
Morrison, Hilary G.;McArthur, Andrew G.;Sogin, Mitchell L.
通讯作者：
Sogin, Mitchell L.