Developmentally regulated cleavage of tRNAs in the bacterium Streptomyces coelicolor.
Developmentally regulated cleavage of tRNAs in the bacterium Streptomyces coelicolor.
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DOI：
10.1093/nar/gkm1096
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发表时间：
2008-02
影响因子：
14.9
通讯作者：
Elliot MA
中科院分区：
文献类型：
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作者：
Haiser HJ;Karginov FV;Hannon GJ;Elliot MA
感知和响应环境和生理信号的能力对于土壤中革兰氏阳性细菌天蓝色链霉菌的生存至关重要。营养缺乏引发了一个复杂的形态分化过程，涉及气生菌丝的提高和孢子链的形成，并与临床相关的抗生素和其他次级代谢产物的生产的多样化的数组。这些过程受到严格调控;然而，涉及的基因和信号尚未完全阐明。在这里，我们报告了一种新的tRNA切割事件，遵循相同的时间调节的形态和生理分化，是生长介质依赖性。所有的tRNA似乎都对切割敏感;然而，对于那些指定高度利用的密码子的tRNA，似乎存在增加切割的倾向。与在真核生物中观察到的相反，S.腔棘鱼不受氨基酸饥饿的显著影响，也不受严格反应的诱导或核糖体功能的抑制的影响。在气生发育和抗生素生产中有缺陷的突变体相对于野生型菌株表现出改变的tRNA切割谱。
The ability to sense and respond to environmental and physiological signals is critical for the survival of the soil-dwelling Gram-positive bacterium Streptomyces coelicolor. Nutrient deprivation triggers the onset of a complex morphological differentiation process that involves the raising of aerial hyphae and formation of spore chains, and coincides with the production of a diverse array of clinically relevant antibiotics and other secondary metabolites. These processes are tightly regulated; however, the genes and signals involved have not been fully elucidated. Here, we report a novel tRNA cleavage event that follows the same temporal regulation as morphological and physiological differentiation, and is growth medium dependent. All tRNAs appear to be susceptible to cleavage; however, there appears to be a bias towards increased cleavage of those tRNAs that specify highly utilized codons. In contrast to what has been observed in eukaryotes, accumulation of tRNA halves in S. coelicolor is not significantly affected by amino acid starvation, and is also not affected by induction of the stringent response or inhibition of ribosome function. Mutants defective in aerial development and antibiotic production exhibit altered tRNA cleavage profiles relative to wild-type strains.
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影响因子：
3.6
作者：
Hagege, JM;Cohen, SN
通讯作者：
Cohen, SN
影响因子：
3.6
作者：
Capstick, David S.;Willey, Joanne M.;Elliot, Marie A.
通讯作者：
Elliot, Marie A.
影响因子：
3.2
作者：
Eccleston, M;Ali, RA;Nodwell, J
通讯作者：
Nodwell, J
影响因子：
3.2
作者：
Hunt, AC;Servín-González, L;Buttner, MJ
通讯作者：
Buttner, MJ
PPGPP合成在形态分化和抗生素产生中的全球作用在Coelicolor A3（2）中。
影响因子：
12.3
作者：
通讯作者：
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