The study of two barley type I-like MADS-box genes as potential targets of epigenetic regulation during seed development.
The study of two barley type I-like MADS-box genes as potential targets of epigenetic regulation during seed development.
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在种子发育过程中将两种类似I型的I型MADS-box基因作为表观遗传调节的潜在靶标的研究。
DOI：
10.1186/1471-2229-12-166
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发表时间：
2012-09-17
影响因子：
5.3
通讯作者：
Tsaftaris AS
中科院分区：
文献类型：
--
作者：
Kapazoglou A;Engineer C;Drosou V;Kalloniati C;Tani E;Tsaballa A;Kouri ED;Ganopoulos I;Flemetakis E;Tsaftaris AS
MADS-box基因是一个转录因子大家族，在植物营养和生殖发育过程中起着重要的调控作用。II型MADS盒基因已被深入研究，主要参与营养和开花发育。越来越多的拟南芥I型MADS-box基因的研究，已指定这些基因在配子和种子发育中的关键作用，并已证明，一些I型MADS-box基因的表观遗传调控的DNA甲基化和组蛋白修饰。然而，关于大麦和小麦等重要农艺谷物的报告很少。在这里，我们报告了两个I型MADS盒基因的鉴定和表征，从大麦（大麦），单子叶谷类作物的高农艺重要性。蛋白质序列分析和系统发育分析表明，推测的蛋白质与I型MADS-box蛋白相关，并将其归类于一个独特的谷类分支。这两个基因在种子发育阶段和不同种子大小的大麦品种之间的基因表达显着差异。这些基因之一被证明是由种子发育和应力相关激素阿坝和JA，而在原位杂交本地化的其他基因特定的胚乳亚室诱导。后者的基因组结构与谷类I型MADS盒同源物具有高度保守性，并且这两个基因的染色体位置接近与种子品质性状相关的标记。大麦I型样MADS盒基因的上游和下游调节区在两个不同的发育阶段以及对阿坝处理的响应中存在DNA甲基化差异，这可能与基因表达差异有关。研究了与I型MADS盒基因相关的两个大麦MADS盒基因。这两个基因在不同的种子发育阶段以及在不同种子大小的大麦品种中的差异表达是有证据的。发现这两个大麦I型MADS-box基因受阿坝和JA诱导。DNA甲基化在种子不同发育阶段和外源阿坝处理后的差异提示基因表达的表观遗传调控。大麦I型MADS盒基因的研究扩展了我们对单子叶作物大麦胚乳和种子发育过程中基因调控的研究。
MADS-box genes constitute a large family of transcription factors functioning as key regulators of many processes during plant vegetative and reproductive development. Type II MADS-box genes have been intensively investigated and are mostly involved in vegetative and flowering development. A growing number of studies of Type I MADS-box genes in Arabidopsis, have assigned crucial roles for these genes in gamete and seed development and have demonstrated that a number of Type I MADS-box genes are epigenetically regulated by DNA methylation and histone modifications. However, reports on agronomically important cereals such as barley and wheat are scarce. Here we report the identification and characterization of two Type I-like MADS-box genes, from barley (Hordeum vulgare), a monocot cereal crop of high agronomic importance. Protein sequence and phylogenetic analysis showed that the putative proteins are related to Type I MADS-box proteins, and classified them in a distinct cereal clade. Significant differences in gene expression among seed developmental stages and between barley cultivars with varying seed size were revealed for both genes. One of these genes was shown to be induced by the seed development- and stress-related hormones ABA and JA whereas in situ hybridizations localized the other gene to specific endosperm sub-compartments. The genomic organization of the latter has high conservation with the cereal Type I-like MADS-box homologues and the chromosomal position of both genes is close to markers associated with seed quality traits. DNA methylation differences are present in the upstream and downstream regulatory regions of the barley Type I-like MADS-box genes in two different developmental stages and in response to ABA treatment which may be associated with gene expression differences. Two barley MADS-box genes were studied that are related to Type I MADS-box genes. Differential expression in different seed developmental stages as well as in barley cultivars with different seed size was evidenced for both genes. The two barley Type I MADS-box genes were found to be induced by ABA and JA. DNA methylation differences in different seed developmental stages and after exogenous application of ABA is suggestive of epigenetic regulation of gene expression. The study of barley Type I-like MADS-box genes extends our investigations of gene regulation during endosperm and seed development in a monocot crop like barley.
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大米中的MADS-box基因家族：在生殖发育和压力期间，全基因组识别，组织和表达分析。
影响因子：
4.4
作者：
Arora, Rita;Agarwal, Pinky;Ray, Swatismita;Singh, Ashok Kumar;Singh, Vijay Pal;Tyagi, Akhilesh K.;Kapoor, Sanjay
通讯作者：
Kapoor, Sanjay
影响因子：
9.5
作者：
Bauer MJ;Fischer RL
通讯作者：
Fischer RL
影响因子：
11.4
作者：
Gramzow, Lydia;Ritz, Markus S.;Theissen, Gunter
通讯作者：
Theissen, Gunter
影响因子：
7.2
作者：
Colombo, Monica;Masiero, Simona;Colombo, Lucia
通讯作者：
Colombo, Lucia
DOI：
10.1126/science.1190614
发表时间：
2010-10-29
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Feng S;Jacobsen SE;Reik W
通讯作者：
Reik W