YopR impacts type III needle polymerization in Yersinia species.
YopR impacts type III needle polymerization in Yersinia species.
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DOI：
10.1111/j.1365-2958.2009.06988.x
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发表时间：
2010-01
影响因子：
3.6
通讯作者：
Schneewind O
中科院分区：
文献类型：
--
作者：
Blaylock B;Berube BJ;Schneewind O
耶尔森氏菌III型机器的标志是存在从细菌表面延伸的针。针有两个功能，一是作为将效应物输送到免疫细胞的管道，二是作为传感器。聚合的针蛋白，YscF，被认为是感知环境钙离子的阈值水平，以触发分泌。yopR（yscH）是yscEFG下游的基因，编码蛋白伴侣和针的主要结构单元。在这里，我们研究了YopR对III型分泌和发病机制的贡献。缺乏yopR的鼠疫耶尔森氏菌KIM D27突变体在败血性鼠疫小鼠模型中的毒力缺陷。小肠结肠炎耶尔森氏菌W22703的yopR变体显示出将效应物注射到巨噬细胞中的能力降低，并且与野生型耶尔森氏菌相比，需要更低的钙浓度来激活III型分泌。此外，yopR突变体不能将YscF组装成针状复合物，而是将YscF分泌到培养基中。这些结果意味着YopR可能参与控制YscF的分泌，从而影响III型机器的组装。另一种可能性，即YopR直接参与YscF的聚合，似乎不太可能，因为YopR与纯化的针状物无关。
A hallmark of Yersinia type III machines is the presence of needles extending from the bacterial surface. Needles perform two functions, serving as the conduits for the transport of effectors into immune cells but also acting as a sensor. The polymerized needle protein, YscF, is thought to perceive threshold levels of environmental calcium ions to trigger secretion. yopR (yscH) is a gene downstream of yscEFG, encoding the chaperones and principal building blocks of the needle. Here we investigated the contribution of YopR towards type III secretion and pathogenesis. Yersinia pestis KIM D27 mutants lacking yopR were defective for virulence in a mouse model of septicemic plague. yopR variants of Yersinia enterocolitica W22703 displayed a reduced ability to inject effectors into macrophages and required lower calcium concentrations to activate type III secretion than wild-type yersiniae. Further, yopR mutants failed to assemble YscF into needle complexes and instead secreted YscF into the medium. These results imply that YopR may be involved in controlling the secretion of YscF, thereby impacting the assembly of type III machines. An alternative possibility, that YopR participates directly in the polymerization of YscF, seems less likely as YopR is not associated with purified needles.
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影响因子：
3.6
作者：
Lee, VT;Anderson, DM;Schneewind, O
通讯作者：
Schneewind, O
影响因子：
3.6
作者：
Agrain, C;Sorg, I;Cornelis, GR
通讯作者：
Cornelis, GR
DOI：
10.1073/pnas.071065798
发表时间：
2001-04-10
影响因子：
11.1
作者：
Hoiczyk, E;Blobel, G
通讯作者：
Blobel, G
影响因子：
3.6
作者：
Lee, VT;Schneewind, O
通讯作者：
Schneewind, O
影响因子：
3.1
作者：
DeBord, Kristin L.;Anderson, Deborah M.;Schneewind, Olaf
通讯作者：
Schneewind, Olaf