The Predatory Myxobacterium Citreicoccus inhibens gen. nov. sp. nov. Showed Antifungal Activity and Bacteriolytic Property against Phytopathogens.
The Predatory Myxobacterium Citreicoccus inhibens gen. nov. sp. nov. Showed Antifungal Activity and Bacteriolytic Property against Phytopathogens.
复制标题
捕食性柠檬粘杆菌抑制菌属。
DOI：
10.3390/microorganisms9102137
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发表时间：
2021-10-12
期刊：
影响因子：
4.5
通讯作者：
Zhu H
中科院分区：
文献类型：
--
作者：
Zhou Y;Yi S;Zang Y;Yao Q;Zhu H
生物防治剂在田间防治效果差、性能不稳定，限制了其应用和推广。筛选具有较高拮抗活性、适应性强、田间存活率高的微生物是今后的研究方向。粘细菌是一种适应性很广的土壤捕食性细菌，被认为是很好的拮抗菌。采用大肠杆菌诱食法从我国南方亚热带森林土壤中分离到一株粘细菌M34。根据形态观察、生理试验、生化特征、16 S rRNA基因序列和基因组数据，菌株M34被鉴定为一个新属和新种，代表粘球菌科的一个新分支，建议将其命名为抑制柠檬球菌gen. nov. sp. nov.（模式菌株M34 T = GDMCC 1.2275 T = KCTC 82453 T）。显微观察表明，M34的典型特征，包括子实体的形成和细胞外纤维的相互连接，有助于细胞在不同环境中的适应。此外，该菌株对植物病原真菌表现出一定的抗真菌活性，对革兰氏阴性和革兰氏阳性植物病原细菌表现出一定的捕食活性。其生物保护机制主要是由于硝吡咯及其衍生物的抗真菌活性和胞外蛋白对病原菌的裂解活性。由于其多种有益性状，菌株M34有潜力被开发成用于管理真菌和细菌植物病原体的通用生防剂。
The application and promotion of biological control agents are limited because of poor efficacy and unstable performance in the field. Screening microorganisms with high antagonistic activity, effective adaptability, and high field-survival should be prospected. Myxobacteria are soil predatory bacteria with wide adaptability, which are considered as good antagonists. Here, we report a myxobacterium strain M34 isolated from subtropical forest soil in South China using the Escherichia coli baiting method. Based on the morphological observation, physiological test, biochemical characteristics, 16S rRNA gene sequence, and genomic data, strain M34 was identified as a novel genus and novel species, representing a new clade of Myxococcaceae, for which the name Citreicoccus inhibens gen. nov. sp. nov. is proposed (type strain M34T = GDMCC 1.2275T = KCTC 82453T). The typical features of M34, including fruiting body formation and extracellular fibrillar interconnection, indicated by microscopic observations, contributed to cell adaption in different environments. Furthermore, the strain showed antifungal activity against phytopathogenic fungi and predatory activity to both Gram-negative and Gram-positive phytopathogenic bacteria. The bioprotective mechanisms are attributed to the presence of pyrrolnitrin and derivative with antifungal activity and the extracellular proteins with lytic activity against pathogenic bacteria. Due to its multiple beneficial traits, strain M34 has the potential to be developed into a versatile biocontrol agent for the management of both fungal and bacterial phytopathogens.
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影响因子：
2.9
作者：
Chavan, S. B.;Deshpande, M. V.
通讯作者：
Deshpande, M. V.
影响因子：
11.3
作者：
Berleman JE;Kirby JR
通讯作者：
Kirby JR
影响因子：
4.1
作者：
Fira, Djordje;Dimkic, Ivica;Stankovic, Slavisa
通讯作者：
Stankovic, Slavisa
DOI：
10.1007/978-3-030-35955-3_5
发表时间：
2020-01-01
期刊：
PLANT DISEASE MANAGEMENT STRATEGIES FOR SUSTAINABLE AGRICULTURE THROUGH TRADITIONAL AND MODERN APPROACHES
影响因子：
--
作者：
Iftikhar, Yasir;Sajid, Ashara;Ul Haq, Zia
通讯作者：
Ul Haq, Zia
影响因子：
1.7
作者：
Bankevich, Anton;Nurk, Sergey;Pevzner, Pavel A.
通讯作者：
Pevzner, Pavel A.