Architecture of the Atg17 complex as a scaffold for autophagosome biogenesis.
Architecture of the Atg17 complex as a scaffold for autophagosome biogenesis.
复制标题
ATG17复合物的结构是自噬体生物发生的支架。
DOI：
10.1016/j.cell.2012.11.028
复制
发表时间：
2012-12-21
期刊：
影响因子：
64.5
通讯作者：
Hurley JH
中科院分区：
文献类型：
--
作者：
Ragusa MJ;Stanley RE;Hurley JH
Macroautophagy is a bulk clearance mechanism in which the double-membraned phagophore grows and engulfs cytosolic material. In yeast, the phagophore nucleates from a cluster of 20-30 nm diameter Atg9-containing vesicles located at a multiprotein assembly known as the preautophagosomal structure (PAS). The crystal structure of a 2:2:2 complex of the earliest-acting PAS proteins, Atg17, Atg29, and Atg31, was solved at 3.05 Å resolution. Atg17 is crescent-shaped with a 10 nm radius of curvature. Dimerization of the Atg17-Atg31-Atg29 complex is critical for both PAS formation and autophagy and each dimer contains two separate and complete crescents. Upon induction of autophagy, Atg17-Atg31-Atg29 assembles with Atg1 and Atg13, which in turn initiates the formation of the phagophore. The C-terminal EAT domain of Atg1 was shown to sense membrane curvature, dimerize, and tether lipid vesicles. These data suggest a structural mechanism for the organization of Atg9 vesicles into the early phagophore.
登录
查看更多内容
DOI：
10.1083/jcb.200901145
发表时间：
2009-05-18
期刊：
The Journal of cell biology
影响因子：
--
作者：
Klemm RW;Ejsing CS;Surma MA;Kaiser HJ;Gerl MJ;Sampaio JL;de Robillard Q;Ferguson C;Proszynski TJ;Shevchenko A;Simons K
通讯作者：
Simons K
影响因子：
3.3
作者：
Jung, Chang Hwa;Jun, Chang Bong;Kim, Do-Hyung
通讯作者：
Kim, Do-Hyung
DOI：
10.1016/j.bbrc.2007.02.150
发表时间：
2007-05-04
影响因子：
3.1
作者：
Kabeya, Yukiko;Kawarnata, Tomoko;Ohsumi, Yoshinori
通讯作者：
Ohsumi, Yoshinori
影响因子：
20.3
作者：
Liu, Fei;Lee, Jae Y.;Guan, Jun-Lin
通讯作者：
Guan, Jun-Lin
影响因子：
6.1
作者：
Konarev, PV;Volkov, VV;Svergun, DI
通讯作者：
Svergun, DI