Segregation of sphingolipids and sterols during formation of secretory vesicles at the trans-Golgi network.
Segregation of sphingolipids and sterols during formation of secretory vesicles at the trans-Golgi network.
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DOI：
10.1083/jcb.200901145
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发表时间：
2009-05-18
期刊：
影响因子：
--
通讯作者：
Simons K
中科院分区：
文献类型：
--
作者：
Klemm RW;Ejsing CS;Surma MA;Kaiser HJ;Gerl MJ;Sampaio JL;de Robillard Q;Ferguson C;Proszynski TJ;Shevchenko A;Simons K
高尔基体反式网络（trans-Golgi network，TGN）是真核细胞分泌途径中的主要分选站。TGN如何分类蛋白质和脂质以在质膜上产生鞘脂和甾醇的富集尚不清楚。为了解决这一基本问题，在膜贩运，我们设计了一个免疫隔离程序的特异性恢复后高尔基体分泌囊泡运输跨膜筏蛋白从TGN的细胞表面的酵母酿酒酵母。使用一种新的定量鸟枪脂质组学方法，我们可以证明，TGN分选选择性地丰富麦角固醇和鞘脂种类的免疫分离的分泌囊泡。这一发现首次表明TGN具有分选膜脂质的能力。此外，观察到免疫隔离的囊泡表现出更高的膜顺序比后期高尔基体膜，通过C-Laurdan分光光度法测量，强烈表明，脂筏发挥作用的TGN分选机械。
The trans-Golgi network (TGN) is the major sorting station in the secretory pathway of all eukaryotic cells. How the TGN sorts proteins and lipids to generate the enrichment of sphingolipids and sterols at the plasma membrane is poorly understood. To address this fundamental question in membrane trafficking, we devised an immunoisolation procedure for specific recovery of post-Golgi secretory vesicles transporting a transmembrane raft protein from the TGN to the cell surface in the yeast Saccharomyces cerevisiae. Using a novel quantitative shotgun lipidomics approach, we could demonstrate that TGN sorting selectively enriched ergosterol and sphingolipid species in the immunoisolated secretory vesicles. This finding, for the first time, indicates that the TGN exhibits the capacity to sort membrane lipids. Furthermore, the observation that the immunoisolated vesicles exhibited a higher membrane order than the late Golgi membrane, as measured by C-Laurdan spectrophotometry, strongly suggests that lipid rafts play a role in the TGN-sorting machinery.
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影响因子：
11.4
作者：
Gurunathan, S;David, D;Gerst, JE
通讯作者：
Gerst, JE
影响因子：
11.4
作者：
Holthuis, JCM;Nichols, BJ;Pelham, HRB
通讯作者：
Pelham, HRB
DOI：
10.1073/pnas.0511136103
发表时间：
2006-02-21
影响因子：
11.1
作者：
Brügger, B;Glass, B;Kräusslich, HG
通讯作者：
Kräusslich, HG
影响因子：
4.8
作者：
Gaigg, B;Timischl, B;Schneiter, R
通讯作者：
Schneiter, R
影响因子：
2.3
作者：
Ejsing, CS;Moehring, T;Shevchenko, A
通讯作者：
Shevchenko, A