Gonadogenesis analysis and sex differentiation in cultured turbot (Scophthalmus maximus)
Gonadogenesis analysis and sex differentiation in cultured turbot (Scophthalmus maximus)
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养殖大菱鲆的性腺发生分析和性别分化
DOI：
10.1007/s10695-016-0284-5
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发表时间：
2016-09
影响因子：
2.9
通讯作者：
Li Jun
中科院分区：
文献类型：
--
作者：
Zhao Chunyan;Xu Shihong;Liu Yifan;Wang Yanfeng;Liu Qinghua;Li Jun
大菱鲆（schophthalmus maximus）作为一种比目鱼，在生长速度和性成熟方面具有很强的性别二态性，是最重要的具有重要商业价值的养殖鱼类之一。本研究从组织学角度详细介绍了大菱鲆性别分化过程中生殖细胞增殖、迁移和性腺早期发育的基本信息。到15ádays孵化后（dph），有6 ~ 9个具有大细胞核的原始生殖细胞（PGCs）。位于肾系管下的PGCs沿背肠系膜向生殖嵴区域从0到15ádph迁移。在迁移过程中，PGCs的数量保持不变，vasa的表达没有明显变化。在20ádph，生殖嵴处的体细胞聚集表明初生性腺的形成。PGCs的数量增加到60个，vasa的表达首次上调。未分化性腺在形态上无性别分化迹象，随着生殖细胞和体细胞数量/大小从20增加到35ádph，性腺逐渐变大。36-52ádph期间，观察性腺细胞学分化。在假定的I型性腺组织睾丸中（呈长矛状），生殖细胞的数量稳步增加，生殖细胞的特征与以前相同。同时，在II型性腺组织（棒状）的推定卵巢中，生殖细胞在两种不同的生殖细胞群中增殖和诱导。一种类型的生殖细胞形态特征为未分化的生殖细胞，而另一种类型的生殖细胞进行有丝分裂，大小较小，细胞核斑驳。在60ádph，在II型的性腺中存在卵巢腔，它将发育成卵巢。然而，直到90ádph才在I型性腺中发现精原囊肿，这表明睾丸形成。
As a flatfish, the turbot (Scophthalmus maximus) is one of the most important farmed fish species with great commercial value, which has a strong sexual dimorphism on growth rate and sexual maturity. In this study, using histology, the basic information on proliferation and migration of germ cells and early gonadal development during sex differentiation in turbot were described in detail. There were six to nine individual primordial germ cells (PGCs) with large nuclei until 15ádays post-hatching (dph). The PGCs located under the mesonephric ducts undergo migration along the dorsal mesentery toward the region of the genital ridge from 0 to 15ádph. During migration, the number of PGCs was constant, and the expression ofvasahad no significant changes. At 20ádph, the aggregation of somatic cells at genital ridge indicated the formation of primary gonad. Furthermore, the number of PGCs was increased to 60 and the expression ofvasawas upregulated for the first time. The undifferentiated gonads with no morphological indications of sex differentiation grew larger with the increase in germ cells and somatic cells number/size from 20 to 35ádph. During 36–52ádph, cytological gonadal differentiation was observed. In presumptive testes of type I gonadal tissue (with a lance shape), the number of germ cells increased steadily and the germ cells had the same characteristics as before. Meanwhile, in presumptive ovaries of type II gonadal tissue (with a club-like shape), the germ cells proliferated and induced in two different populations of germ cells. One type had the morphological characteristics as undifferentiated germ cells, while the other type of germ cells underwent mitosis exhibiting smaller size and mottled nuclei. At 60ádph, ovarian cavity was present in the gonad of type II, which would develop into ovaries. However, spermatogonial cysts were not noticed in the gonad of type I until 90ádph, which indicated the formation of the testes.
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DOI：
--
发表时间：
1972-10
期刊：
Journal of embryology and experimental morphology
影响因子：
--
作者：
N. Satoh;N. Egami
通讯作者：
N. Satoh;N. Egami
影响因子：
3.7
作者：
Boysen C;Davis EG;Beard LA;Lubbers BV;Raghavan RK
通讯作者：
Raghavan RK
DOI：
--
发表时间：
2002-03
期刊：
The Journal of experimental biology
影响因子：
--
作者：
D. Uchida;M. Yamashita;T. Kitano;T. Iguchi
通讯作者：
D. Uchida;M. Yamashita;T. Kitano;T. Iguchi
DOI：
--
发表时间：
1985-12
期刊：
Journal of embryology and experimental morphology
影响因子：
--
作者：
H. Parmentier;L. Timmermans
通讯作者：
H. Parmentier;L. Timmermans
影响因子：
2.6
作者：
Arezo, M. J.;D'Alessandro, S.;Berois, N.
通讯作者：
Berois, N.