Reciprocal Effect of Copper and Iron Regulation on the Proteome of Synechocystis sp. PCC 6803.
Reciprocal Effect of Copper and Iron Regulation on the Proteome of Synechocystis sp. PCC 6803.
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DOI：
10.3389/fbioe.2021.673402
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发表时间：
2021
影响因子：
5.7
通讯作者：
Wang YC
中科院分区：
文献类型：
--
作者：
Zhen ZH;Qin S;Ren QM;Wang Y;Ma YY;Wang YC
蓝细菌可以通过金属稳态来适应环境中铜和铁浓度的变化，但解释其动态关系的一般机制很少。在这项研究中，我们评估了集胞藻PCC 6803的生长和叶绿素荧光，并研究了蛋白质组学对铜和铁的扣除。结果表明，铜和铁在不同浓度组合下对集胞藻6803的生长和光合作用具有交互作用。一些参与铜、铁吸收和光合电子传递系统的蛋白质也表现出Cu-Fe蛋白质组关联。铜、铁胁迫下蛋白质丰度的变化影响了集胞藻6803的光合电子活性，进而影响其生长和光合作用。基于这些结果，我们推测集胞藻6803的Cu-Fe蛋白质组学关联可以通过外膜-周质空间-内质膜-类囊体膜的摄取系统来阐明，并且这种关联主要是维持电子传递所必需的。本研究提供了一个更广泛的观点，关于蛋白质组学之间的关联铜和铁在蓝藻，这将有助于阐明这两种金属元素在蓝藻的能量代谢和生物量积累的作用。
Cyanobacteria can acclimate to changing copper and iron concentrations in the environment via metal homeostasis, but a general mechanism for interpreting their dynamic relationships is sparse. In this study, we assessed growth and chlorophyll fluorescence of Synechocystis sp. PCC 6803 and investigated proteomic responses to copper and iron deductions. Results showed that copper and iron exerted reciprocal effect on the growth and photosynthesis of Synechocystis sp. PCC 6803 at combinations of different concentrations. And some proteins involved in the uptake of copper and iron and the photosynthetic electron transport system exhibit Cu–Fe proteomic association. The protein abundance under copper and iron deduction affected the photosynthetic electronic activity of Synechocystis sp. PCC 6803 and eventually affected the growth and photosynthesis. Based on these results, we hypothesize that the Cu–Fe proteomic association of Synechocystis sp. PCC 6803 can be elucidated via the uptake system of outer membrane-periplasmic space-inner plasma membrane-thylakoid membrane, and this association is mainly required to maintain electron transfer. This study provides a broader view regarding the proteomic association between Cu and Fe in cyanobacteria, which will shed light on the role of these two metal elements in cyanobacterial energy metabolism and biomass accumulation.
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影响因子：
7.4
作者：
BURNAP, RL;TROYAN, T;SHERMAN, LA
通讯作者：
SHERMAN, LA
影响因子：
3.7
作者：
Fraser JM;Tulk SE;Jeans JA;Campbell DA;Bibby TS;Cockshutt AM
通讯作者：
Cockshutt AM
影响因子：
5
作者：
Holland, HD
通讯作者：
Holland, HD
影响因子：
2.1
作者：
HUTBER, GN;HUTSON, KG;ROGERS, LJ
通讯作者：
ROGERS, LJ
DOI：
10.1093/bfgp/elm030
发表时间：
2007-12-01
期刊：
Briefings in Functional Genomics & Proteomics
影响因子：
--
作者：
De la Cerda, Berta;Castielli, Ornella;De la Rosa, Miguel A.
通讯作者：
De la Rosa, Miguel A.