Novel stem/progenitor cell population from murine tracheal submucosal gland ducts with multipotent regenerative potential.
Novel stem/progenitor cell population from murine tracheal submucosal gland ducts with multipotent regenerative potential.
复制标题
DOI：
10.1002/stem.680
复制
发表时间：
2011-08
期刊：
影响因子：
5.2
通讯作者：
Gomperts, Brigitte N.
中科院分区：
文献类型：
--
作者：
Hegab, Ahmed E.;Ha, Vi Luan;Gilbert, Jennifer L.;Zhang, Kelvin Xi;Malkoski, Stephen P.;Chon, Andy T.;Darmawan, Daphne O.;Bisht, Bharti;Ooi, Aik T.;Pellegrini, Matteo;Nickerson, Derek W.;Gomperts, Brigitte N.
关键词：
气道上皮与环境直接接触，因此始终面临受伤的风险。基底细胞（BC）被发现可以修复表面上皮（SE），但其他干细胞群对气道上皮修复的贡献尚未确定。我们证明，除了 BC 之外，气道粘膜下腺 (SMG) 导管细胞也能在严重缺氧缺血性损伤中幸存下来。我们开发了一种将导管细胞与气道隔离的方法。使用体外和体内模型来比较导管细胞和BC的自我更新和分化潜力。我们发现只有导管细胞能够再生 SMG 肾小管和导管，以及覆盖 SMG 的 SE。因此，SMG 导管细胞是用于气道上皮修复的多能干细胞，这对于肺再生领域非常重要，因为确定修复细胞群可能有助于识别气道疾病患者的新治疗靶点和基于细胞的疗法。
The airway epithelium is in direct contact with the environment and therefore constantly at risk for injury. Basal cells (BCs) have been found to repair the surface epithelium (SE), but the contribution of other stem cell populations to airway epithelial repair has not been identified. We demonstrated that airway submucosal gland (SMG) duct cells, in addition to BCs, survived severe hypoxic-ischemic injury. We developed a method to isolate duct cells from the airway. In vitro and in vivo models were used to compare the self-renewal and differentiation potential of duct cells and BCs. We found that only duct cells were capable of regenerating SMG tubules and ducts, as well as the SE overlying the SMGs. SMG duct cells are therefore a multipotent stem cell for airway epithelial repair This is of importance to the field of lung regeneration as determining the repairing cell populations could lead to the identification of novel therapeutic targets and cell-based therapies for patients with airway diseases.
登录
查看更多内容
DOI：
10.1152/ajplung.00169.2002
发表时间：
2002-12-01
影响因子：
4.9
作者：
You, YJ;Richer, EJ;Brody, SL
通讯作者：
Brody, SL
影响因子：
11.2
作者：
Ooi AT;Mah V;Nickerson DW;Gilbert JL;Ha VL;Hegab AE;Horvath S;Alavi M;Maresh EL;Chia D;Gower AC;Lenburg ME;Spira A;Solis LM;Wistuba II;Walser TC;Wallace WD;Dubinett SM;Goodglick L;Gomperts BN
通讯作者：
Gomperts BN
影响因子：
14.8
作者：
Huang, Da Wei;Sherman, Brad T.;Lempicki, Richard A.
通讯作者：
Lempicki, Richard A.
影响因子：
56.9
作者：
REYNOLDS, BA;WEISS, S
通讯作者：
WEISS, S
影响因子：
6
作者：
Hong, KU;Reynolds, SD;Stripp, BR
通讯作者：
Stripp, BR