Background-Free Proton NMR Spectroscopy with Radiofrequency Amplification by Stimulated Emission Radiation.
Background-Free Proton NMR Spectroscopy with Radiofrequency Amplification by Stimulated Emission Radiation.
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DOI：
10.1002/anie.202108939
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发表时间：
2021-12-06
期刊：
影响因子：
--
通讯作者：
Chekmenev EY
中科院分区：
文献类型：
--
作者：
Joalland B;Theis T;Appelt S;Chekmenev EY
我们报道了利用受激发射辐射（RASER）的射频放大技术对超极化生物分子进行无背景质子检测的应用。对~0.3 M乙酸乙酯进行了超极化。采用质量因子Q = 68的标准（非低温）电感检测器，在临床相关磁场1.4 T下，无射频激励检测信噪比超过100,000的质子核磁共振信号。从质子化溶剂（甲醇）或其他添加的辅助溶剂（如乙醇、水或牛血清）中未观察到质子背景信号。此外，我们证明了激光检测没有射频激发的生物流体中的一团超极化造影剂。生物介质中超极化造影剂的完全无背景质子检测为高分辨率核磁共振光谱和体内光谱和成像领域的新应用铺平了道路。在质子化溶剂和生物流体的极端情况下，受激辐射射频放大（RASER）被用于超极化核磁共振的无背景检测。这种方法为高分辨率核磁共振和体内核磁共振在超极化分子质子检测中的广泛应用铺平了道路。
We report on the utility of Radiofrequency Amplification by Stimulated Emission Radiation (RASER) for background-free proton detection of hyperpolarized biomolecules. We performed hyperpolarization of ~0.3 M ethyl acetate via pairwise parahydrogen addition to vinyl acetate. Proton NMR signal with signal-to-noise ratio exceeding 100,000 was detected without radio-frequency excitation at the clinically relevant magnetic field of 1.4 T using a standard (non-cryogenic) inductive detector with quality factor of Q = 68. No proton background signal was observed from protonated solvent (methanol) or other added co-solvents such as ethanol, water or bovine serum. Moreover, we demonstrate RASER detection without radio-frequency excitation of a bolus of hyperpolarized contrast agent in biological fluid. Completely background-free proton detection of hyperpolarized contrast agents in biological media paves the way to new applications in the areas of high-resolution NMR spectroscopy and in vivo spectroscopy and imaging. Radiofrequency Amplification by Stimulated Emission Radiation (RASER) was employed for background-free detection of hyperpolarized NMR resonances even in the extreme case of protonated solvents and biological fluids. This approach paves the way to a wide range of high-resolution NMR- and in vivo MRI applications of proton detection of hyperpolarized molecules.
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DOI：
10.1002/anie.202108306
发表时间：
2021-09-13
期刊：
Angewandte Chemie (International ed. in English)
影响因子：
--
作者：
Korchak S;Kaltschnee L;Dervisoglu R;Andreas L;Griesinger C;Glöggler S
通讯作者：
Glöggler S
影响因子：
2.2
作者：
Coffey, Aaron M.;Truong, Milton L.;Chekmenev, Eduard Y.
通讯作者：
Chekmenev, Eduard Y.
影响因子：
2.2
作者：
Birchall, Jonathan R.;Irwin, Robert K.;Chekmenev, Eduard Y.
通讯作者：
Chekmenev, Eduard Y.
影响因子：
2.2
作者：
Chen, Hsueh-Ying;Lee, Youngbok;Hilty, Christian
通讯作者：
Hilty, Christian
影响因子：
3.3
作者：
Fekete, Marianna;Ahwal, Fadi;Duckett, Simon B.
通讯作者：
Duckett, Simon B.