Orthogonal design for optimization of pigment extraction from surface sediments of the Changjiang River Estuary
Orthogonal design for optimization of pigment extraction from surface sediments of the Changjiang River Estuary
复制标题
长江口表层沉积物色素提取优化正交设计
DOI：
10.1007/s13131-011-0131-6
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发表时间：
2011-09
影响因子：
1.4
通讯作者：
Yao Peng
中科院分区：
文献类型：
--
作者：
Thomas S.;Bianchi;Yu Zhigang;Zhao Jun;Yao Peng
使用合适的溶剂从沉积物中提取色素进行高效液相色谱分析，对于获得植物浮游和底栖藻类生物量以及群落组成的定性和定量估计至关重要。采用L9（34）正交试验设计，对长江三角洲前缘河口沉积物色素提取的5个影响因素（样品脱水量、提取溶剂、提取时间、提取次数和溶剂体积与样品重量之比）进行了研究。结果表明，沉积色素的最佳提取方法应包括在提取前将样品冷冻干燥。通过L9（34）正交试验，对各因素对沉积色素提取的影响进行了分离，结果表明：提取溶剂对沉积色素提取的影响最大，提取时间次之，提取次数和料液比最小。极性色素的提取以丙酮-甲醇-水（体积比80：15：5）混合溶剂最好，非极性色素的提取以100%丙酮为佳。对于本研究中使用的大多数颜料（即，多甲藻黄素、岩藻黄素、四黄素、硅藻黄质、玉米黄质、脱镁叶绿素-A和β-胡萝卜素），发现3 h是从这些三角洲沉积物中提取的足够时间。然而，叶绿素-a，最重要的色素用于估计藻类生物量，需要12小时。少量的溶剂（3 ml）重复提取获得最大量和多样性的色素。遗憾的是，没有发现适合于沉积物中所有色素的提取方法。提取程序的选择应根据每项研究的目的，并考虑到有关沉积物和色素的性质。
Using a suitable solvent for extracting pigments from sediment for high performance liquid chromatography (HPLC) analysis is critical for obtaining qualitative and quantitative estimates of phytoplanktonic and benthic algal biomass, as well as community composition. Five methodological factors (sample dehydration, extraction solvent, extraction duration, number of extractions, and ratio of solvent volume: sample weight) were studied using an L9(34) orthogonal design in a sedimentary pigment extraction experiment on samples collected from the Changjiang large-river delta-front estuary (LDE), using HPLC analysis. The results show that the optimal extraction method for sedimentary pigments should include freeze-drying samples prior to extraction. The effects of different factors on sedimentary pigment extraction were separated by the L9(34) orthogonal design experiments and showed that the extraction solvent was the most important, with extraction duration the second most important, and numbers of extraction and ratio of solvent volume: sample weight was the least important. The mixed solvent treatment comprised of acetone, methanol and water (80:15:5, by volume) was best for polar pigment extraction, with 100% acetone better for apolar pigments. For most pigments employed in this study (i.e., peridinin, fucoxanthin, alloxanthin, diatoxanthin, zeaxanthin, pheophytin-a andβ-carotene), 3 h was found to be enough time for extraction from these deltaic sediments. However, for chlorophyll-a, the most important pigment used for estimating algal biomass, 12 h was needed. A small amount of solvent (3 ml) with duplicate extractions obtained the greatest amount and diversity of pigments. Unfortunately, no extraction method was found to be suitable for all pigments in sediments. The choice of extraction procedure should be made in accordance with the objective of each study, taking into consideration the properties of sediments and pigments in question.
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影响因子：
2.3
作者：
C. Naimie;C. Blain;D. Lynch
通讯作者：
C. Naimie;C. Blain;D. Lynch
DOI：
10.4319/lom.2005.3.477
发表时间：
2005-10
期刊：
Limnology and Oceanography: Methods
影响因子：
--
作者：
Nina S. Reuss;D. Conley
通讯作者：
Nina S. Reuss;D. Conley
影响因子：
3
作者：
J. Louda;Joseph W. Loitz;D. Rudnick;E. W. Baker
通讯作者：
J. Louda;Joseph W. Loitz;D. Rudnick;E. W. Baker
影响因子：
6.1
作者：
Devesa, R.;Moldes, A.;Barral, M. T.
通讯作者：
Barral, M. T.
影响因子：
2.5
作者：
C. Lucas;P. Holligan
通讯作者：
C. Lucas;P. Holligan