Bacterial colonization reprograms the neonatal gut metabolome.
Bacterial colonization reprograms the neonatal gut metabolome.
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细菌定植会重新编程新生儿肠道代谢组。
DOI：
10.1038/s41564-020-0694-0
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发表时间：
2020-06
影响因子：
28.3
通讯作者：
Wu GD
中科院分区：
文献类型：
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作者：
Bittinger K;Zhao C;Li Y;Ford E;Friedman ES;Ni J;Kulkarni CV;Cai J;Tian Y;Liu Q;Patterson AD;Sarkar D;Chan SHJ;Maranas C;Saha-Shah A;Lund P;Garcia BA;Mattei LM;Gerber JS;Elovitz MA;Kelly A;DeRusso P;Kim D;Hofstaedter CE;Goulian M;Li H;Bushman FD;Zemel BS;Wu GD
人类婴儿肠道中最初的微生物定植和后来的演替与以后的健康和疾病有关。第一个肠道微生物组出现的时间，以及对早期生命代谢组的影响，刚刚开始被定义。在这里，我们评估了88名非洲裔美国新生儿的肠道微生物组，蛋白质组和代谢组，使用生命最初几天收集的粪便样本。肠道细菌在出生后16小时就可以用分子方法检测出来。对三种最常见的菌种（大肠杆菌、粪肠球菌和普通拟杆菌）的详细分析并未表明新生儿肠道定植的基因组特征。细菌的出现与大约50种人类蛋白质的丰度降低、游离氨基酸水平降低以及细菌发酵产物（包括乙酸盐和琥珀酸盐）的增加有关。使用通量平衡建模和体外实验，我们提供的证据表明，发酵的氨基酸提供了一种机制的初始生长的大肠杆菌，最常见的早期殖民者，在厌氧条件下。这些结果提供了人类肠道中第一批微生物的深入表征，并显示了它们的外观如何改变生物化学环境。
Initial microbial colonization and later succession in the gut of human infants are linked to health and disease later in life. The timing of the appearance of the first gut microbiome, and the consequences for the early life metabolome, are just starting to be defined. Here we evaluated the gut microbiome, proteome, and metabolome in 88 African American newborns using fecal samples collected in the first few days of life. Gut bacteria became detectable using molecular methods by 16 hours after birth. Detailed analysis of the three most common species, Escherichia coli, Enterococcus faecalis, and Bacteroides vulgatus, did not suggest a genomic signature for neonatal gut colonization. The appearance of bacteria was associated with reduced abundance of approximately 50 human proteins, decreased levels of free amino acids, and an increase in products of bacterial fermentation, including acetate and succinate. Using flux balance modeling and in vitro experiments, we provide evidence that fermentation of amino acids provides a mechanism for the initial growth of Escherichia coli, the most common early colonizer, under anaerobic conditions. These results provide a deep characterization of the first microbes in the human gut and show how the biochemical environment is altered by their appearance.
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影响因子：
16.6
作者：
Durack J;Kimes NE;Lin DL;Rauch M;McKean M;McCauley K;Panzer AR;Mar JS;Cabana MD;Lynch SV
通讯作者：
Lynch SV
影响因子：
7.4
作者：
Cai J;Zhang J;Tian Y;Zhang L;Hatzakis E;Krausz KW;Smith PB;Gonzalez FJ;Patterson AD
通讯作者：
Patterson AD
影响因子：
3.7
作者：
Hu J;Nomura Y;Bashir A;Fernandez-Hernandez H;Itzkowitz S;Pei Z;Stone J;Loudon H;Peter I
通讯作者：
Peter I
影响因子：
5.2
作者：
Dobbler PT;Procianoy RS;Mai V;Silveira RC;Corso AL;Rojas BS;Roesch LFW
通讯作者：
Roesch LFW
影响因子：
--
作者：
Clasquin, Michelle F;Melamud, Eugene;Rabinowitz, Joshua D
通讯作者：
Rabinowitz, Joshua D