Dynamics of individual polymers using microfluidic based microcurvilinear flow.
Dynamics of individual polymers using microfluidic based microcurvilinear flow.
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使用基于微流体的微曲线流的单个聚合物的动力学。
DOI：
10.1039/b907860f
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发表时间：
2009
期刊：
影响因子：
6.1
通讯作者：
Leduc,PhilipR
中科院分区：
文献类型：
--
作者：
Cheng,Chao-Min;Kim,Yongtae;Yang,Jui-Ming;Leuba,SanfordH;Leduc,PhilipR
高分子动力学在材料科学、物理学、生物学和医学等领域发挥着重要作用。单个分子（如生物聚合物）的时空响应对于新材料的开发、对细胞结构（包括细胞骨架动力学）的扩展理解以及DNA复制至关重要。然而，探测单分子动力学的能力往往受到小规模技术的限制，这些技术可以操纵这些系统来揭示高度复杂的行为。微米和纳米级技术的进步同时为我们提供了宝贵的工具，可以与这些系统，包括微流体等方法接口。在这里，我们报告通过一个小规模的流体方法，我们已被用来施加一个基于流动的高径向加速度（103 g）对个别灵活的聚合物上的微曲线流的创建。我们能够采用这种基于微流体的方法来调节和控制流速和加速度，以观察我们设备中荧光标记的λ-噬菌体DNA分子的实时动态。这使我们能够通过简单直接的技术方法在局部区域对单个分子施加机械刺激，包括拉伸和弯曲。我们发现，灵活的DNA分子表现出多模态响应，包括不同的构象和可控的曲率，这些特性直接相关的拉伸和弯曲动力学的曲线流内的位置决定。我们分析了这些单个分子的动力学，以确定它们在该系统中不同位置的伸长应变率和曲率（Δ 0.09 µm−1），以探测单个聚合物的结构响应。这些结果表明，我们有能力创造高径向加速度流，并观察直接施加到单个DNA分子的实时动态响应。这种方法也可以用于研究其他生物基聚合物，包括额外的核酸，肌动蛋白丝和微管，并提供一个平台，以了解小规模的柔性聚合物的材料特性。
Polymer dynamics play an important role in a diversity of fields including materials science, physics, biology and medicine. The spatiotemporal responses of individual molecules such as biopolymers have been critical to the development of new materials, the expanded understanding of cell structures including cytoskeletal dynamics, and DNA replication. The ability to probe single molecule dynamics however is often limited by the availability of small-scale technologies that can manipulate these systems to uncover highly intricate behaviors. Advances in micro- and nano-scale technologies have simultaneously provided us with valuable tools that can interface with these systems including methods such as microfluidics. Here, we report on the creation of micro-curvilinear flow through a small-scale fluidic approach, which we have been used to impose a flow-based high radial acceleration (∼103 g) on individual flexible polymers. We were able to employ this microfluidic-based approach to adjust and control flow velocity and acceleration to observe real-time dynamics of fluorescently labeled λ-phage DNA molecules in our device. This allowed us to impose mechanical stimulation including stretching and bending on single molecules in localized regimes through a simple and straightforward technology-based method. We found that the flexible DNA molecules exhibited multimodal responses including distinct conformations and controllable curvatures; these characteristics were directly related to both the elongation and bending dynamics dictated by their locations within the curvilinear flow. We analyzed the dynamics of these individual molecules to determine their elongation strain rates and curvatures (∼0.09 µm−1) at different locations in this system to probe the individual polymer structural response. These results demonstrate our ability to create high radial acceleration flow and observe real-time dynamic responses applied directly to individual DNA molecules. This approach may also be useful for studying other biologically based polymers including additional nucleic acids, actin filaments, and microtubules and provide a platform to understand the material properties of flexible polymers at a small scale.
影响因子：
2.3
作者：
Chandrasekaran, A.;Packirisamy, M.
通讯作者：
Packirisamy, M.
DNA 分子与绝缘柱的电泳碰撞。
影响因子：
8.6
作者：
G. Randall;P. Doyle
通讯作者：
P. Doyle
影响因子：
56.9
作者：
SMITH, SB;FINZI, L;BUSTAMANTE, C
通讯作者：
BUSTAMANTE, C