Cell Sorting in Hydra vulgaris Arises from Differing Capacities for Epithelialization between Cell Types.
Cell Sorting in Hydra vulgaris Arises from Differing Capacities for Epithelialization between Cell Types.
复制标题
DOI：
10.1016/j.cub.2020.07.035
复制
发表时间：
2020-10-05
期刊：
影响因子：
--
通讯作者：
McKinley KL
中科院分区：
文献类型：
--
作者：
Skokan TD;Vale RD;McKinley KL
水螅显示出一种非凡的能力，可以从无序的细胞集合中重组其身体计划。重组开始于将两种上皮细胞类型（内胚层和外胚层）分别分选为内层和外层。区分外胚层和内胚层谱系以驱动分选的细胞特征和行为尚未完全阐明。为了剖析这一过程，我们使用显微操作将不同谱系的单细胞定位在定义的多细胞聚集体表面上，并通过实时成像监测分选结果。虽然分选以前被归因于上皮谱系之间的内在差异，但我们发现所有谱系的单个细胞都向外胚层聚集体的内部分选，包括单个外胚层细胞。这表明，同一谱系的细胞在作为个体或集体分类时可以采取相反的位置。外胚层细胞集体通过集体扩散行为迅速重组上皮，吞噬粘附在其表面的细胞，从而在聚集的外部获得它们的位置。相反，聚集的内胚层细胞持续丧失上皮特征。这些非上皮化的聚集体，就像所有谱系的分离细胞一样，是被外胚层上皮吞噬的粘附旅客。我们发现，九头蛇的局部损伤过程中也会出现外胚层的集体扩散和内胚层的持续去上皮化，这表明九头蛇的伤口愈合和自组织能力可能采用类似的机制。总之，我们的数据表明，不同的上皮化倾向可以将细胞类型分类到不同的区室中，以建立和恢复复杂的组织结构。Skokan等人剖析了Hydra细胞分选背后的细胞行为。快速恢复上皮单层的细胞通过吞噬非上皮化的聚集体和单个细胞而采用外部位置。对局部损伤的类似反应表明伤口愈合和自组织中恢复区隔化的共同机制。
Hydra vulgaris exhibits a remarkable capacity to reassemble its body plan from a disordered aggregate of cells. Reassembly begins by sorting two epithelial cell types, endoderm and ectoderm, into inner and outer layers, respectively. The cellular features and behaviors that distinguish ectodermal and endodermal lineages to drive sorting have not been fully elucidated. To dissect this process, we use micromanipulation to position single cells of diverse lineages on the surface of defined multicellular aggregates and monitor sorting outcomes by live imaging. Although sorting has previously been attributed to intrinsic differences between the epithelial lineages, we find that single cells of all lineages sort to the interior of ectodermal aggregates, including single ectodermal cells. This reveals that cells of the same lineage can adopt opposing positions when sorting as individuals or a collective. Ectodermal cell collectives adopt their position at the aggregate exterior by rapidly reforming an epithelium that engulfs cells adhered to its surface through a collective spreading behavior. In contrast, aggregated endodermal cells persistently lose epithelial features. These non-epithelialized aggregates, like isolated cells of all lineages, are adherent passengers for engulfment by the ectodermal epithelium. We find that collective spreading of the ectoderm and persistent de-epithelialization in the endoderm also arise during local wounding in Hydra, suggesting that Hydra’s wound-healing and self-organization capabilities may employ similar mechanisms. Together, our data suggest that differing propensities for epithelialization can sort cell types into distinct compartments to build and restore complex tissue architecture. Skokan et al. dissect the cellular behaviors underlying cell sorting in Hydra. Cells that rapidly restore an epithelial monolayer adopt exterior positions by engulfing non-epithelialized aggregates and single cells. Similar responses to local damage suggest common mechanisms for restoring compartmentalization in wound healing and self-organization.
登录
查看更多内容
DOI：
10.1038/newbio239098a0
发表时间：
1972-01-01
期刊：
NATURE-NEW BIOLOGY
影响因子：
--
作者：
GIERER, A;BODE, H;FLICK, K
通讯作者：
FLICK, K
影响因子：
9.2
作者：
Landsberg, Katharina P.;Farhadifar, Reza;Dahmann, Christian
通讯作者：
Dahmann, Christian
影响因子：
3.4
作者：
Cochet-Escartin, Olivier;Locke, Tiffany T.;Collins, Eva-Maria S.
通讯作者：
Collins, Eva-Maria S.
影响因子：
10.7
作者：
Hemmrich, Georg;Khalturin, Konstantin;Bosch, Thomas C. G.
通讯作者：
Bosch, Thomas C. G.
DOI：
10.3791/51888
发表时间：
2014-09-11
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Juliano, Celina E;Lin, Haifan;Steele, Robert E
通讯作者：
Steele, Robert E