Development of pathological diagnostics of human kidney cancer by multiple staining using new fluorescent Fluolid dyes.
Development of pathological diagnostics of human kidney cancer by multiple staining using new fluorescent Fluolid dyes.
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DOI：
10.1155/2014/437871
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发表时间：
2014
影响因子：
--
通讯作者：
Kiyama R
中科院分区：
文献类型：
--
作者：
Wuxiuer D;Zhu Y;Ogaeri T;Mizuki K;Kashiwa Y;Nishi K;Isobe S;Aoyagi T;Kiyama R
新型荧光 Fluolid 染料具有优于其他染料的优点，例如对热、干燥和强光的稳定性。在这里，我们使用针对 Kank1、细胞角蛋白 7 (CK7) 和 CD10 的一抗对肾肿瘤、透明细胞肾细胞癌 (RCC)、乳头状肾细胞癌、嫌色细胞肾细胞癌、获得性囊性疾病相关肾细胞癌 (ACD-RCC) 和肾血管平滑肌脂肪瘤 (AML) 进行同步免疫染色，并用二抗检测这些抗体 分别用 Fluolid-Orange、Fluolid-Green 和 Alexa Fluor 647 标记。 Kank1 在正常肾小管、乳头状 RCC 和 ACD-RCC 中染色，在所有其他肿瘤中呈弱染色或阴性。正常肾小管、乳头状肾细胞癌和 ACD-RCC 中 CK7 呈阳性。相反，CD10 在肾小管和透明细胞 RCC、乳头状 RCC、AML 和 AC-RCC 中表达，在嫌色细胞 RCC 中表达较弱。这些结果可能有助于区分肾肿瘤和肾细胞癌亚型。我们还检查了荧光的稳定性，发现组织切片和在室温下保存近三年后的同一切片之间的 Fluolid 染料的荧光图像是相同的。这表明组织切片经多种荧光标记染色后可以在室温下保存相对较长的时间，这为病理诊断打开了一扇大门。
New fluorescent Fluolid dyes have advantages over others such as stability against heat, dryness, and excess light. Here, we performed simultaneous immunostaining of renal tumors, clear cell renal cell carcinoma (RCC), papillary RCC, chromophobe RCC, acquired cystic disease-associated RCC (ACD-RCC), and renal angiomyolipoma (AML), with primary antibodies against Kank1, cytokeratin 7 (CK7), and CD10, which were detected with secondary antibodies labeled with Fluolid-Orange, Fluolid-Green, and Alexa Fluor 647, respectively. Kank1 was stained in normal renal tubules, papillary RCC, and ACD-RCC, and weakly or negatively in all other tumors. CK7 was positive in normal renal tubules, papillary RCC, and ACD-RCC. In contrast, CD10 was expressed in renal tubules and clear cell RCC, papillary RCC, AML, and AC-RCC, and weakly in chromophobe RCC. These results may contribute to differentiating renal tumors and subtypes of RCCs. We also examined the stability of fluorescence and found that fluorescent images of Fluolid dyes were identical between a tissue section and the same section after it was stored for almost three years at room temperature. This indicates that tissue sections can be stored at room temperature for a relatively long time after they are stained with multiple fluorescent markers, which could open a door for pathological diagnostics.
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影响因子：
1.8
作者：
Ahn, Soomin;Kwon, Ghee Young;Shim, Jung Won
通讯作者：
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影响因子：
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作者：
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作者：
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Kiyama, R
DOI：
10.1097/pai.0b013e31823fecd3
发表时间：
2012-10-01
影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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