Regulation of Colonic Mucosal MicroRNA Expression via Multiple Targets in Visceral Hypersensitivity Rats by Tongxieyaofang.
Regulation of Colonic Mucosal MicroRNA Expression via Multiple Targets in Visceral Hypersensitivity Rats by Tongxieyaofang.
复制标题
通过Tongxieyaofang在内脏超敏反应中，通过多个靶标调节结肠粘膜microRNA的表达。
DOI：
10.3349/ymj.2018.59.8.945
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发表时间：
2018-10
影响因子：
2.4
通讯作者：
Zhang S
中科院分区：
文献类型：
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作者：
Chao G;Wang Y;Ye F;Zhang S
本研究旨在筛选内脏高敏感大鼠结肠差异表达的microRNAs，建立其表达谱，探讨痛泻要方治疗肠易激综合征的作用机制。将40只SD大鼠随机分为4组：对照组、模型对照组(直肠刺激诱发腹壁收缩反应)、治疗对照组(生理盐水)、痛泻要方治疗组(痛泻要方)。我们利用液体芯片技术筛选结肠黏膜miRNAs的差异表达，并用逆转录-聚合酶链式反应(RT-PCR)验证其表达。成功建立内脏高敏感大鼠模型。我们发现，在内脏高敏感大鼠，let-7f、let-7i、miR-130b、miR-29a、miR-132、miR-21和miR-375的表达上调(p<；0.05)，而miR-24、miR-31a、miR-192、miR-221和miR-223的表达下调(p<；0.05)。痛泻要方治疗后，与治疗对照组相比，let-7f、let-7i、miR-130b、miR-29a、miR-132、miR-21和miR-375的表达降低(p<；0.05)，而miR-24、miR-31a、miR-192、miR-221、miR-223的表达增加(p<；0.05)。MiRNAs在内脏高敏感性中起关键作用，可能成为痛泻要方治疗IBS的靶点。
This study aimed to screen for differentially expressed microRNAs (miRNAs) in the colons of rats with visceral hypersensitivity to build the expression profiles of miRNAs therein and to determine the mechanism of Tongxieyaofang use in the treatment of irritable bowel syndrome (IBS). Forty Sprague-Dawley rats were divided randomly into four groups: control group, model control group (induced by rectum stimulus and evaluated by abdominal withdraw reaction), treatment control group (normal saline), and Tongxieyaofang group (treated with Tongxieyaofang). We screened for differential expression of colonic mucosal miRNAs using liquid chip technology and verified the expression thereof using reverse transcription-PCR. The visceral hypersensitivity rat model was successfully established. We found the expression of let-7f, let-7i, miR-130b, miR-29a, miR-132, miR-21, and miR-375 to be up-regulated (p<0.05), while the expression of miR-24, miR-31a, miR-192, miR-221, and miR-223 was down-regulated (p<0.05) in the visceral hypersensitivity rats. After treatment with Tongxieyaofang, the expression of let-7f, let-7i, miR-130b, miR-29a, miR-132, miR-21, and miR-375 was reduced (p<0.05), whereas the expression of miR-24, miR-31a, miR-192, miR-221, miR-223 was increased, compared to the treatment control group (p<0.05). MiRNAs play a pivotal role in visceral hypersensitivity and might be targets in the treatment of IBS by Tongxieyaofang.
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影响因子：
56.9
作者：
Farh, KKH;Grimson, A;Bartel, DP
通讯作者：
Bartel, DP
影响因子：
3.4
作者：
Davoodian, Nahid;Lotfi, Abbas S.;Arjmand, Sare
通讯作者：
Arjmand, Sare
影响因子：
5.8
作者：
Mussnich, Paula;Rosa, Roberta;D'Angelo, Daniela
通讯作者：
D'Angelo, Daniela
影响因子：
4.9
作者：
Lin, Xu-tao;Zheng, Xiao-bin;He, Xiao-sheng
通讯作者：
He, Xiao-sheng
阐明Danzhixiaoyao WAN及其成分草药对抗氧化活性和一氧化氮产生的抑制作用。
影响因子：
--
作者：
Liao, Hui;Banbury, Linda K.;Leach, David N.
通讯作者：
Leach, David N.