Elucidation of danzhixiaoyao wan and its constituent herbs on antioxidant activity and inhibition of nitric oxide production.
Elucidation of danzhixiaoyao wan and its constituent herbs on antioxidant activity and inhibition of nitric oxide production.
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阐明Danzhixiaoyao WAN及其成分草药对抗氧化活性和一氧化氮产生的抑制作用。
DOI：
10.1093/ecam/nel091
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发表时间：
2007-12
影响因子：
--
通讯作者：
Leach, David N.
中科院分区：
文献类型：
--
作者：
Liao, Hui;Banbury, Linda K.;Leach, David N.
关键词：
丹栀逍遥丸（DW）是中国常见的10味中药制剂，用于调节影响妇女的几种临床病症。本研究试图从体外药理学的角度来解释DW的泻热作用。采用氧自由基吸收能力（ORAC）测定法比较全方和各单味药的抗氧化活性，采用Griess测定法比较全方和各单味药对脂多糖（LPS）激活的RAW 264.7巨噬细胞产生一氧化氮（NO）的抑制作用。结果表明，DW作为一个整体配方既有抗氧化活性，也有抑制NO产生的IE，而DW的单个中药成分在其ORAC值和抑制NO产生方面存在差异。整个DW的ORAC值为450 μmol TE g−1。单味草药的抗氧化活性顺序为：薄荷（1352 μmol TE g−1）>甘草（1184 μmol TE g − 1）>栀子（1129 μmol TE g − 1）>牡丹（465 μmol TE g −1），其贡献是相加的而不是协同的。M可显著抑制刺激的RAW264.7小鼠巨噬细胞（0.5 ± 0.1 μM vs 38.9 ± 2.3 μM）产生亚硝酸盐（P < 0.05）。haplocalyx、单萼棉G. jasminoides、北柴胡和芍药。DW作为一个整体对NO生产有IE，但这并不重要。单味药M. Haplocalyx的ORAC值和IE值最高，其次是G.茉莉花这两种中药均具有中医理论中的泻热作用，其泻热作用可能与抗氧化活性和抑制NO生成有关。
Danzhixiaoyao Wan (DW) is a common 10 herbs formulation in China for regulating several clinical conditions affecting women. This research tried to explain one of DW's functions, purging heat, using in vitro pharmacological analyses. The whole formulation and each single herb of DW were compared based on antioxidant activity with the oxygen radical absorbance capacity (ORAC) assay, and for their inhibitory effect (IE) on nitric oxide (NO) production by lipopolysaccharide (LPS)-activated RAW 264.7 macrophages with the Griess assay. The results showed that DW as a whole formulation had both antioxidant activity and an IE on NO production, while the individual herb component of DW varied in their ORAC values and inhibition of NO production. The ORAC value of the whole DW was 450 μmol TE g−1. The order of antioxidant (ORAC) activity of the single herbs was: Mentha haplocalyx (1352 μmol TE g−1) > Glycyrrhiza uralensis (1184 μmol TE g−1) > Gardenia jasminoides (1129 μmol TE g−1) > Paeonia suffruticosa (465 μmol TE g−1), with the contributions being additive rather than synergistic. The production of nitrite by stimulated RAW 264.7 murine macrophages (unstimulated: 0.5 ± 0.1 μM versus LPS: 38.9 ± 2.3 μM) was significantly inhibited (P < 0.05) by M. haplocalyx, G. jasminoides, Bupleurum chinense and Paeonia lactiflora. DW as a whole had an IE on NO production, but this was not significant. The single herb M. haplocalyx had the highest ORAC value and the highest IE on NO production, followed by G. jasminoides. Both of these herbs have the ‘purging heat’ property in the theory of traditional Chinese medicine and this property of the samples may be correlated with the antioxidant activity and IE on NO production.
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影响因子：
2.9
作者：
GREEN, LC;WAGNER, DA;TANNENBAUM, SR
通讯作者：
TANNENBAUM, SR
影响因子：
6.1
作者：
Pham, TQ;Cormier, F;Van Calsteren, MR
通讯作者：
Van Calsteren, MR
DOI：
10.1073/pnas.91.6.2046
发表时间：
1994-03-15
影响因子：
11.1
作者：
VANE, JR;MITCHELL, JA;WILLOUGHBY, DA
通讯作者：
WILLOUGHBY, DA
Kampo公式Keishibukuryogan（Gui-Zhi-Fu-ling-wan）在类风湿关节炎患者中降低了疾病活性和可溶性血管粘附分子-1。
影响因子：
--
作者：
Nozaki, Kazuya;Hikiami, Hiroaki;Goto, Hirozo;Nakagawa, Takako;Shibahara, Naotoshi;Shimada, Yutaka
通讯作者：
Shimada, Yutaka
影响因子：
6.1
作者：
Ou, BX;Hampsch-Woodill, M;Prior, RL
通讯作者：
Prior, RL