Nucleosome Dynamics during Transcription Elongation.
Nucleosome Dynamics during Transcription Elongation.
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DOI：
10.1021/acschembio.0c00617
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发表时间：
2020-12-18
影响因子：
4
通讯作者：
Lee TH
中科院分区：
文献类型：
--
作者：
Huynh MT;Yadav SP;Reese JC;Lee TH
核小体是真核生物基因组的基本包装单位。核小体中DNA和组蛋白之间的动态相互作用是基因可及性调节的分子基础，基因可及性调节支配各种DNA模板化过程的动力学，例如RNA聚合酶II（Pol II）的转录延伸。基于化学修饰的组蛋白的单分子FRET测量，我们研究了转录延伸过程中的核小体动力学以及它如何受到组蛋白乙酰化H3 K56和组蛋白伴侣Nap 1的影响，这两者都可以影响DNA-组蛋白相互作用。我们观察到H3 K56乙酰化显著缩短了Pol II在核小体进入区域附近的停顿时间，而Nap 1没有引起明显的差异。我们还发现Pol II通过核小体的延伸率不受乙酰化或Nap 1的影响。这些结果表明，H3 K56乙酰化通过帮助暂停的Pol II恢复而促进Pol II通过核小体的易位，并且Nap 1不影响Pol II的进展。转录后，只有一小部分核小体保持完整，不受H3 K56乙酰化或Nap 1的影响。这些结果表明，（i）自发的核小体开放使Pol II进展，（ii）Pol II介导核小体重组非常低效，和（iii）Nap 1在没有其他因素的情况下不促进核小体解体或重组在转录过程中。
The nucleosome is the basic packing unit of the eukaryotic genome. Dynamic interactions between DNA and histones in the nucleosome are the molecular basis of gene accessibility regulation that governs the kinetics of various DNA-templated processes such as transcription elongation by RNA Polymerase II (Pol II). Based on single-molecule FRET measurements with chemically modified histones, we investigated the nucleosome dynamics during transcription elongation and how it is affected by histone acetylation at H3 K56 and the histone chaperone Nap1, both of which can affect DNA-histone interactions. We observed that H3K56 acetylation dramatically shortens the pause duration of Pol II near the entry region of the nucleosome, while Nap1 induces no noticeable difference. We also found that the elongation rate of Pol II through the nucleosome is unaffected by the acetylation or Nap1. These results indicate that H3K56 acetylation facilitates Pol II translocation through the nucleosome by assisting paused Pol II to resume and that Nap1 does not affect Pol II progression. Following transcription, only a small fraction of nucleosomes remain intact, which is unaffected by H3K56 acetylation or Nap1. These results suggest that (i) spontaneous nucleosome opening enables Pol II progression, (ii) Pol II mediates nucleosome reassembly very inefficiently, and (iii) Nap1 in the absence of other factors does not promote nucleosome disassembly or reassembly during transcription.
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DOI：
10.1021/acs.jpcb.5b09734
发表时间：
2015-12-03
期刊：
The journal of physical chemistry. B
影响因子：
--
作者：
Kim J;Lee J;Lee TH
通讯作者：
Lee TH
影响因子：
16
作者：
Bednar, J;Studitsky, VM;Woodcock, CL
通讯作者：
Woodcock, CL
影响因子：
16.8
作者：
Bilokapic S;Strauss M;Halic M
通讯作者：
Halic M
影响因子：
2.9
作者：
Arimura, Yasuhiro;Tachiwana, Hiroaki;Kurumizaka, Hitoshi
通讯作者：
Kurumizaka, Hitoshi
影响因子：
64.5
作者：
JOHNSON, TL;CHAMBERLIN, MJ
通讯作者：
CHAMBERLIN, MJ