The Rvv two-component regulatory system regulates biofilm formation and colonization in Vibrio cholerae.
The Rvv two-component regulatory system regulates biofilm formation and colonization in Vibrio cholerae.
复制标题
DOI：
10.1371/journal.ppat.1011415
复制
发表时间：
2023-05
期刊：
影响因子：
6.7
通讯作者：
中科院分区：
文献类型：
--
作者：
兼性人类病原体霍乱弧菌采用双组分信号转导系统（TCS）来感知和响应其感染周期中遇到的环境信号。TCS由传感器组氨酸激酶（HK）和反应调节因子（RR）组成;霍乱弧菌基因组编码43个HK和49个RR，其中25个预计是同源对。使用每个HK基因的缺失突变体，我们分析了vpsL，弧菌多糖和生物膜形成所需的生物膜基因的转录。我们发现以前没有研究过的霍乱弧菌TCS（现在称为Rvv）控制生物膜基因转录。Rvv TCS是存在于30%的弧菌目物种中的三基因操纵子的一部分。rvv操纵子编码RvvA（HK）; RvvB（同源RR）;和RvvC（一种功能未知的蛋白质）。rvvA的缺失增加了生物膜基因的转录并改变了生物膜的形成，而rvvB或rvvC的缺失导致生物膜基因转录没有变化。在ΔrvvA中观察到的表型取决于RvvB。突变RvvB以模拟RR的组成型活性和非活性版本仅影响ΔrvvA遗传背景中的表型。在RvvA激酶活性所需的保守残基突变不影响表型，而磷酸酶活性所需的保守残基的突变模仿的rvvA突变体的表型。此外，ΔrvvA具有明显的定殖缺陷，其定殖缺陷依赖于RvvB和RvvB的磷酸化状态，而不依赖于VPS的产生。我们发现RvvA的磷酸酶活性调节生物膜基因转录、生物膜形成和定植表型。这是第一次系统分析霍乱弧菌HKs在生物膜基因转录中的作用，并鉴定了一种新的生物膜形成和毒力调节因子，促进了我们对TCS在调节霍乱弧菌这些关键细胞过程中所起作用的理解。双组分信号转导系统是微生物最常用的调节网络之一，用于感知、响应和适应环境，并增加其环境适应性。我们系统地分析了霍乱弧菌HKs，并鉴定了Rvv，一种新的生物膜基因转录和毒力调节因子。本研究为深入了解霍乱弧菌环境适应性的信号转导途径奠定了基础。此外，它增强了我们对TCS在调节霍乱弧菌中这些关键细胞过程中所起作用的理解。
The facultative human pathogen, Vibrio cholerae, employs two-component signal transduction systems (TCS) to sense and respond to environmental signals encountered during its infection cycle. TCSs consist of a sensor histidine kinase (HK) and a response regulator (RR); the V. cholerae genome encodes 43 HKs and 49 RRs, of which 25 are predicted to be cognate pairs. Using deletion mutants of each HK gene, we analyzed the transcription of vpsL, a biofilm gene required for Vibrio polysaccharide and biofilm formation. We found that a V. cholerae TCS that had not been studied before, now termed Rvv, controls biofilm gene transcription. The Rvv TCS is part of a three-gene operon that is present in 30% of Vibrionales species. The rvv operon encodes RvvA, the HK; RvvB, the cognate RR; and RvvC, a protein of unknown function. Deletion of rvvA increased transcription of biofilm genes and altered biofilm formation, while deletion of rvvB or rvvC lead to no changes in biofilm gene transcription. The phenotypes observed in ΔrvvA depend on RvvB. Mutating RvvB to mimic constitutively active and inactive versions of the RR only impacted phenotypes in the ΔrvvA genetic background. Mutating the conserved residue required for kinase activity in RvvA did not affect phenotypes, whereas mutation of the conserved residue required for phosphatase activity mimicked the phenotype of the rvvA mutant. Furthermore, ΔrvvA displayed a significant colonization defect which was dependent on RvvB and RvvB phosphorylation state, but not on VPS production. We found that RvvA’s phosphatase activity regulates biofilm gene transcription, biofilm formation, and colonization phenotypes. This is the first systematic analysis of the role of V. cholerae HKs in biofilm gene transcription and resulted in the identification of a new regulator of biofilm formation and virulence, advancing our understanding of the role TCSs play in regulating these critical cellular processes in V. cholerae. Two-component signal transduction systems are one of the most used regulatory networks by microorganisms to sense, respond, and adapt to their environments and increase their environmental fitness. We systematically analyzed V. cholerae HKs and identified Rvv, a new regulator of biofilm gene transcription and virulence. This study lays the groundwork for understanding the signal transduction pathways that regulate V. cholerae’s environmental fitness. Additionally, it enhances our understanding of the role TCSs play in regulating these critical cellular processes in V. cholerae.
登录
查看更多内容
影响因子：
3.6
作者：
Correa, NE;Lauriano, CM;Klose, KE
通讯作者：
Klose, KE
DOI：
10.1073/pnas.2021166117
发表时间：
2020-12-22
影响因子：
11.1
作者：
Bridges AA;Fei C;Bassler BL
通讯作者：
Bassler BL
影响因子：
5.6
作者：
Chamnongpol, S;Groisman, EA
通讯作者：
Groisman, EA
影响因子：
3.6
作者：
Hammer, BK;Bassler, BL
通讯作者：
Bassler, BL
影响因子：
5.1
作者：
Bilecen K;Yildiz FH
通讯作者：
Yildiz FH