Identification of a calcium-controlled negative regulatory system affecting Vibrio cholerae biofilm formation.
Identification of a calcium-controlled negative regulatory system affecting Vibrio cholerae biofilm formation.
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DOI：
10.1111/j.1462-2920.2009.01923.x
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发表时间：
2009-08
影响因子：
5.1
通讯作者：
Yildiz FH
中科院分区：
文献类型：
--
作者：
Bilecen K;Yildiz FH
霍乱弧菌S引起霍乱暴发的能力与其生存能力和对水环境变化的适应能力有关。在霍乱弧菌的自然水生栖息地中，可以改变的环境条件之一是钙(Ca+2)。在这项研究中，我们研究了霍乱弧菌对细胞外钙水平变化的反应。全基因组表达谱显示，钙离子降低了生物膜基质产生所需基因的表达。Luria-Bertani(Luria-Bertani，Luria-Bertani)含钙(LBCa~(2+))的培养基能使霍乱弧菌形成的生物膜厚度减小，粗糙度增加。此外，Ca~(2+)的加入会导致生物膜的溶解。在生长在LBCa+中的细胞中，编码一对双组分调控系统的两个基因(现在被称为钙调节传感器(CARS)和调节器(CARR))的转录减少。对CARS和CARR的零表达和过表达等位基因的分析表明，VPs(弧菌多糖)基因的表达和生物膜的形成受到CARRS双组分调控系统的负调控。通过上位性分析，我们确定了CARR与VPS基因表达的负调控因子HapR平行作用。
Vibrio cholerae ’s capacity to cause outbreaks of cholera is linked to its survival and adaptability to changes in aquatic environments. One of the environmental conditions that can vary in V. cholerae’s natural aquatic habitats is calcium (Ca+2). In this study, we investigated the response of V. cholerae to changes in extracellular Ca2+ levels. Whole-genome expression profiling revealed that Ca2+ decreased the expression of genes required for biofilm matrix production. Luria–Bertani (LB) medium supplemented with Ca2+ (LBCa2+) caused V. cholerae to form biofilms with decreased thickness and increased roughness, as compared with biofilms formed in LB. Furthermore, addition of Ca2+ led to dissolution in biofilms. Transcription of two genes encoding a two-component regulatory system pair, now termed calcium-regulated sensor (carS) and regulator (carR), was decreased in cells grown in LBCa2+. Analysis of null and overexpression alleles of carS and carR revealed that expression of vps (Vibrio polysaccharide) genes and biofilm formation are negatively regulated by the CarRS two-component regulatory system. Through epistasis analysis we determined that CarR acts in parallel with HapR, the negative regulator of vps gene expression.
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影响因子：
3.2
作者：
Beyhan, Sinem;Bilecen, Kivanc;Yildiz, Fitnat H.
通讯作者：
Yildiz, Fitnat H.
影响因子：
3.2
作者：
Eguchi, Y;Okada, T;Utsumi, R
通讯作者：
Utsumi, R
影响因子：
3.6
作者：
Hammer, BK;Bassler, BL
通讯作者：
Bassler, BL
DOI：
10.1073/pnas.2334614100
发表时间：
2003-11-25
影响因子：
11.1
作者：
Kierek, K;Watnick, PI
通讯作者：
Watnick, PI
影响因子：
3.2
作者：
Beyhan, Sinem;Tischler, Anna D.;Yildiz, Fitnat H.
通讯作者：
Yildiz, Fitnat H.