Electromagnetic Controlled Closed-Head Model of Mild Traumatic Brain Injury in Mice.
Electromagnetic Controlled Closed-Head Model of Mild Traumatic Brain Injury in Mice.
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DOI：
10.3791/64556
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发表时间：
2022-09-28
期刊：
影响因子：
--
通讯作者：
Bachstetter AD
中科院分区：
文献类型：
--
作者：
Macheda T;Roberts K;Bachstetter AD
高度可复制的创伤性脑损伤（TBI）动物模型，具有明确的病理，需要测试治疗干预和了解TBI如何改变脑功能的机制。多种脑外伤动物模型的可用性对于模拟人类脑外伤的不同方面和严重程度是必要的。本文描述了使用中线闭合性脑损伤（CHI）来建立轻度TBI小鼠模型。该模型被认为是轻度的，因为它不会产生基于神经成像或总的神经元损失的结构性脑损伤。然而，单次撞击产生的病理足以在损伤后至少1个月测量认知损伤。本文定义了一种利用立体定向电磁冲击器诱导小鼠CHI的逐步方案。轻度中线CHI模型的好处包括损伤引起的变化的可重复性和低死亡率。该模型在损伤后1年内进行了神经影像学、神经化学、神经病理和行为改变的时间表征。该模型是使用相同的冲击器装置控制皮质冲击的开放颅骨模型的补充。因此，实验室可以用相同的冲击器对轻度弥漫性TBI和局灶性中重度TBI进行建模。
Highly reproducible animal models of traumatic brain injury (TBI), with well-defined pathologies, are needed for testing therapeutic interventions and understanding the mechanisms of how a TBI alters brain function. The availability of multiple animal models of TBI is necessary to model the different aspects and severities of TBI seen in people. This manuscript describes the use of a midline closed head injury (CHI) to develop a mouse model of mild TBI. The model is considered mild because it does not produce structural brain lesions based on neuroimaging or gross neuronal loss. However, a single impact creates enough pathology that cognitive impairment is measurable at least 1 month after injury. A step-by-step protocol to induce a CHI in mice using a stereotaxically guided electromagnetic impactor is defined in the paper. The benefits of the mild midline CHI model include the reproducibility of the injury-induced changes with low mortality. The model has been temporally characterized up to 1 year after the injury for neuroimaging, neurochemical, neuropathological, and behavioral changes. The model is complementary to open skull models of controlled cortical impact using the same impactor device. Thus, labs can model both mild diffuse TBI and focal moderate-to-severe TBI with the same impactor.
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DOI：
10.33696/immunology.4.132
发表时间：
2022
期刊：
Journal of cellular immunology
影响因子：
--
作者：
Yanckello, Lucille M;Fanelli, Brian;McCulloch, Scott;Xing, Xin;Sun, McKenna;Hammond, Tyler C;Colwell, Rita;Gu, Zezong;Ericsson, Aaron C;Chang, Ya-Hsuan;Bachstetter, Adam D;Lin, Ai-Ling
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影响因子：
1.2
作者：
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影响因子：
3.7
作者：
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通讯作者：
Bachstetter, Adam D.
影响因子：
4.2
作者：
Petraglia, Anthony L.;Plog, Benjamin A.;Huang, Jason H.
通讯作者：
Huang, Jason H.
影响因子：
34.7
作者：
Xiong, Ye;Mahmood, Asim;Chopp, Michael
通讯作者：
Chopp, Michael