Genome sequencing and assessment of plant growth-promoting properties of a Serratia marcescens strain isolated from vermicompost.
Genome sequencing and assessment of plant growth-promoting properties of a Serratia marcescens strain isolated from vermicompost.
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DOI：
10.1186/s12864-018-5130-y
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发表时间：
2018-10-16
期刊：
影响因子：
4.4
通讯作者：
Venancio TM
中科院分区：
文献类型：
--
作者：
Matteoli FP;Passarelli-Araujo H;Reis RJA;da Rocha LO;de Souza EM;Aravind L;Olivares FL;Venancio TM
植物-细菌联合因其在以可持续的方式提高作物生产力方面的潜力而被广泛研究。粘质沙雷氏菌是肠杆菌科细菌的一种，广泛存在于包括土壤在内的各种环境中。在这里，我们描述了粘质链球菌UENF-22GI的基因组测序和植物生长促进能力的评估，该菌株是从成熟的牛粪蠕虫堆肥中分离出来的。在体外，粘质链霉菌UENF-22GI能够溶解磷和锌，产生吲哚化合物(可能是IAA)，并能定植菌丝和抑制两种植物病原真菌的生长。在温室条件下接种该菌株对玉米幼苗生长和生物量有显著的促进作用。粘质链霉菌UENF-22GI基因组有5个Mb，组装在17个支架上，共4662个基因(4528个是蛋白质编码的基因)。没有鉴定出任何质粒。粘质链球菌UENF-22GI在系统发育上属于一个几乎完全由非临床菌株组成的分支。我们确定了基因和操纵子，这些基因和操纵子可能负责实验中描述的有趣的促进植物生长的特征。粘质链球菌UENF-22GI基因组包含一个水平转移的基因组岛，通过一种新的ADP-核糖基转移酶类蛋白和可能的去氮嘌呤碱基修饰DNA，参与抗生素生产、抗生素耐药性和抗噬菌体防御，这可能有助于其与其他细菌的竞争。综上所述，我们的研究结果表明粘质链霉菌UENF-22GI是一种很好的候选菌株，可用于富集基质以促进植物生长或作为农业生物接种剂的一部分。本文的在线版本(10.1186/s12864-0185130-y)包含补充材料，可供授权用户使用。
Plant-bacteria associations have been extensively studied for their potential in increasing crop productivity in a sustainable manner. Serratia marcescens is a species of Enterobacteriaceae found in a wide range of environments, including soil. Here we describe the genome sequencing and assessment of plant growth-promoting abilities of S. marcescens UENF-22GI, a strain isolated from mature cattle manure vermicompost. In vitro, S. marcescens UENF-22GI is able to solubilize P and Zn, to produce indole compounds (likely IAA), to colonize hyphae and counter the growth of two phytopathogenic fungi. Inoculation of maize with this strain remarkably increased seedling growth and biomass under greenhouse conditions. The S. marcescens UENF-22GI genome has 5 Mb, assembled in 17 scaffolds comprising 4662 genes (4528 are protein-coding). No plasmids were identified. S. marcescens UENF-22GI is phylogenetically placed within a clade comprised almost exclusively of non-clinical strains. We identified genes and operons that are likely responsible for the interesting plant-growth promoting features that were experimentally described. The S. marcescens UENF-22GI genome harbors a horizontally-transferred genomic island involved in antibiotic production, antibiotic resistance, and anti-phage defense via a novel ADP-ribosyltransferase-like protein and possible modification of DNA by a deazapurine base, which likely contributes to its competitiveness against other bacteria. Collectively, our results suggest that S. marcescens UENF-22GI is a strong candidate to be used in the enrichment of substrates for plant growth promotion or as part of bioinoculants for agriculture. The online version of this article (10.1186/s12864-018-5130-y) contains supplementary material, which is available to authorized users.
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