Plant-associated symbiotic Burkholderia species lack hallmark strategies required in mammalian pathogenesis.
Plant-associated symbiotic Burkholderia species lack hallmark strategies required in mammalian pathogenesis.
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DOI：
10.1371/journal.pone.0083779
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Hirsch AM
中科院分区：
文献类型：
--
作者：
Angus AA;Agapakis CM;Fong S;Yerrapragada S;Estrada-de los Santos P;Yang P;Song N;Kano S;Caballero-Mellado J;de Faria SM;Dakora FD;Weinstock G;Hirsch AM
伯克霍尔德氏菌是一个多样化和动态的属，包括致病物种以及与植物形成复杂相互作用的物种。致病性菌株，如假马尔杆菌和马利杆菌，可在哺乳动物中引起严重疾病，而其他伯克霍尔德氏菌菌株是机会性病原体，感染免疫系统受损的人类或动物。虽然已知一些机会性伯克霍尔德菌病原体可以促进植物生长甚至固定氮，但对婴儿、老年人和免疫功能低下者的感染风险不仅导致了对其使用的限制，而且还限制了非致病性共生物种的应用，其中一些物种结瘤豆科植物的根或对植物生长有积极影响。然而，最近的系统发育分析表明，伯克霍尔德氏菌种类分为不同的谱系，这表明在农业环境中安全使用某些共生物种的可能性。一些促进植物生长或降解外源物的环境菌株也包括在共生谱系中。这些物种中的许多都有潜力在肥料不容易获得的地区加强农业，并可能在未来作为接种剂，在受气候变化影响的土壤中生长的作物。在这里，我们利用生物信息学和功能测试解决了几种共生伯克氏菌菌株的致病潜力。利用秀丽隐杆线虫和HeLa细胞进行的一系列感染实验以及致病位点的基因组表征表明，结核杆菌等共生菌株的机会感染风险极低。
Burkholderia is a diverse and dynamic genus, containing pathogenic species as well as species that form complex interactions with plants. Pathogenic strains, such as B. pseudomallei and B. mallei, can cause serious disease in mammals, while other Burkholderia strains are opportunistic pathogens, infecting humans or animals with a compromised immune system. Although some of the opportunistic Burkholderia pathogens are known to promote plant growth and even fix nitrogen, the risk of infection to infants, the elderly, and people who are immunocompromised has not only resulted in a restriction on their use, but has also limited the application of non-pathogenic, symbiotic species, several of which nodulate legume roots or have positive effects on plant growth. However, recent phylogenetic analyses have demonstrated that Burkholderia species separate into distinct lineages, suggesting the possibility for safe use of certain symbiotic species in agricultural contexts. A number of environmental strains that promote plant growth or degrade xenobiotics are also included in the symbiotic lineage. Many of these species have the potential to enhance agriculture in areas where fertilizers are not readily available and may serve in the future as inocula for crops growing in soils impacted by climate change. Here we address the pathogenic potential of several of the symbiotic Burkholderia strains using bioinformatics and functional tests. A series of infection experiments using Caenorhabditis elegans and HeLa cells, as well as genomic characterization of pathogenic loci, show that the risk of opportunistic infection by symbiotic strains such as B. tuberum is extremely low.
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影响因子：
3.2
作者：
DeShazer, D;Brett, PJ;Woods, DE
通讯作者：
Woods, DE
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3.7
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通讯作者：
Lipuma JJ
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Hirsch, Ann M.
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3.1
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3.1
作者：
Essex-Lopresti, AE;Boddey, JA;Titball, RW
通讯作者：
Titball, RW