Inhalation of hydrogen gas ameliorates glyoxylate-induced calcium oxalate deposition and renal oxidative stress in mice.
Inhalation of hydrogen gas ameliorates glyoxylate-induced calcium oxalate deposition and renal oxidative stress in mice.
复制标题
吸入氢气可改善乙醛酸诱导的草酸钙沉积和小鼠肾脏氧化应激。
DOI：
--
复制
发表时间：
2015-03
期刊：
影响因子：
--
通讯作者：
Guo, Zhiyong
中科院分区：
文献类型：
--
作者：
Ye, Zhouheng;Gao, Songyan;Sun, Xuejun;Guo, Zhiyong
本研究旨在探讨氢气（H2）对乙醛酸（乙醛酸）诱导的小鼠肾脏草酸钙（CaOx）结晶沉积的保护作用及其机制。本研究采用乙醛酸（100 mg/kg/d）腹腔注射5 d，建立大鼠肾脏CaOx晶体沉积模型。在给予乙醛酸前两天，开始每天吸入H2 30分钟，并持续7天。实验结束时，收集24 h尿、血清和肾组织进行生化和病理学检测。根据尿钙排泄、肾钙沉积、血清肾损伤分子-1（KIM-1）排泄和TUNEL检测，吸入H2可有效减少CaOx结晶，保护肾脏。肾脏中的晶体沉积与氧化应激相关，其表现为肾脏丙二醛（MDA）和8-羟基脱氧鸟苷（8-OHdG）水平升高以及超氧化物歧化酶（SOD）、谷胱甘肽（GSH）和过氧化氢酶（CAT）活性降低。高剂量H2预处理可逆转这些效应。乙醛酸处理组小鼠肾组织骨桥蛋白（OPN）、CD 44、单核细胞趋化蛋白-1（MCP-1）和白细胞介素-10（IL-10）的表达显著增加，H2可显著抑制OPN、CD 44和MCP-1的表达，但上调IL-10的表达。我们的研究结果表明，吸入H2减少肾结晶，肾氧化损伤和炎症，它可能是一个候选药物，几乎没有不良反应，用于预防肾结石。
The aim of this study is to evaluate the protective effect and underlying mechanism of hydrogen gas (H2) to glyoxylate induced renal calcium oxalate (CaOx) crystal deposition in mice. In present work, rodent renal CaOx crystal deposition model was introduced by intra-abdominal injection of glyoxylate (100 mg/kg/d) for 5 days. Two days before administration of glyoxylate, inhalation of H2 for 30 min per day was initiated and continued for 7 days. By the end of the study, the samples of 24 hours urine, serum and renal tissue were collected for biochemical and pathological assay. According to levels of urine calcium excretion, renal calcium deposition, a serum excretion of kidney injury molecule-1 (KIM-1) assay and a TUNEL assay, inhalation of H2 could successfully decrease the CaOx crystallizations and protect against renal injury. Crystal deposition in the kidneys is associated with oxidative stress, which was indicated by increased levels of renal malondialdehyde (MDA) and 8-hydroxydeoxyguanosine (8-OHdG) and decreased activities of superoxide dismutase (SOD), glutathione (GSH) and catalase (CAT). These effects were reversed by a high-dose H2 pretreatment. The renal expressions of osteopontin (OPN), CD44, monocyte chemoattractant protein-1 (MCP-1) and interleukin-10 (IL-10) were markedly increased in glyoxylate-treated mice, and H2 significantly attenuated the increase of OPN, CD44 and MCP-1 but upregulated the expression of IL-10. Our findings demonstrate that inhalation of H2 reduces renal crystallization, renal oxidative injury and inflammation and it may be a candidate agent with few adverse effects for prevention of nephrolithiasis.
登录
查看更多内容
DOI：
10.1016/s0022-5347(18)38369-1
发表时间：
2004-04
期刊：
Journal of the American Society of Nephrology : JASN
影响因子：
--
作者：
M. Asselman;A. Verhulst;M. de Broe;C. Verkoelen
通讯作者：
M. Asselman;A. Verhulst;M. de Broe;C. Verkoelen
DOI：
10.1007/s10157-004-0292-0
发表时间：
2004-06
期刊：
Journal of Clinical and Experimental Nephrology
影响因子：
--
作者：
Saeed R. Khan
通讯作者：
Saeed R. Khan
白细胞介素10缺乏会加重单侧输尿管梗阻小鼠模型的肾脏炎症和纤维化
影响因子：
5
作者：
Jin, Yuanmeng;Liu, Ruijie;Chen, Nan
通讯作者：
Chen, Nan
影响因子：
--
作者：
Khan, SR
通讯作者：
Khan, SR
DOI：
10.1016/j.bbrc.2007.07.088
发表时间：
2007-09-28
影响因子：
3.1
作者：
Fukuda, Kei-Ichi;Asoh, Sadamitsu;Ohta, Shigeo
通讯作者：
Ohta, Shigeo