Fabrication of a novel hybrid scaffold for tissue engineered heart valve
Fabrication of a novel hybrid scaffold for tissue engineered heart valve
复制标题
用于组织工程心脏瓣膜的新型混合支架的制造
DOI：
10.1007/s11596-009-0513-6
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发表时间：
2009-10
期刊：
影响因子：
--
通讯作者：
Jiawei Shi
中科院分区：
文献类型：
--
作者：
Nianguo Dong;Hao Hong;Jiawei Shi
本研究的目的是利用静电纺丝技术制备生物基质/聚合物杂化支架。然后，将间充质基质细胞(MSCs)种植到支架上，构建组织工程心脏瓣膜。研究了杂化支架对种子细胞增殖、细胞外基质形成和组织工程心脏瓣膜力学性能的影响。骨髓间充质干细胞取自大鼠。将猪主动脉瓣脱细胞，用静电纺丝技术包被聚(3-羟基丁酸酯-4-羟基丁酸酯)包被，再接种并培养14天。对照组将脱细胞瓣膜支架重新接种，并在相同时间内进行培养。对每组标本进行组织学(苏木精-伊红染色、免疫组织化学和扫描电子显微镜)、生化(DNA和4-羟基脯氨酸)和机械学检查。结果表明，细胞再生效果与杂化支架和对照材料相当。混合支架和对照组的样本显示出相同数量的细胞团和4-羟基脯氨酸(P>；0.05)。然而，与对照组相比，杂化支架的试件的机械强度显著增加(P<；0.05)。本研究证实了杂化支架在提高组织工程心脏瓣膜机械强度方面的优越性。与去细胞瓣膜支架相比，杂化支架对间充质干细胞的增殖和细胞外基质的形成具有相似的作用。该复合支架可用于构建组织工程心脏瓣膜。
The aim of this study was to fabricate biomatrix/polymer hybrid scaffolds using an electrospinning technique. Then tissue engineered heart valves were engineered by seeding mesenchymal stromal cells (MSCs) onto the scaffolds. The effects of the hybrid scaffolds on the proliferation of seed cells, formation of extracellular matrix and mechanical properties of tissue engineered heart valves were investigated. MSCs were obtained from rats. Porcine aortic heart valves were decellularized, coated with poly(3-hydroxybutyrate-co-4-hydroxybutyrate) using an electrospinning technique, and reseeded and cultured over a time period of 14 days. In control group, the decellularized valve scaffolds were reseeded and cultured over an equivalent time period. Specimens of each group were examined histologically (hematoxylin-eosin [HE] staining, immunohistostaining, and scanning electron microscopy), biochemically (DNA and 4-hydroxyproline) and mechanically. The results showed that recellularization was comparable to the specimens of hybrid scaffolds and controls. The specimens of hybrid scaffolds and controls revealed comparable amounts of cell mass and 4-hydroxyproline (P>0.05). However, the specimens of hybrid scaffolds showed a significant increase in mechanical strength, compared to the controls (P<0.05). This study demonstrated the superiority of the hybrid scaffolds to increase the mechanical strength of tissue engineered heart valves. And compared to the decellularized valve scaffolds, the hybrid scaffolds showed similar effects on the proliferation of MSCs and formation of extracellular matrix. It was believed that the hybrid scaffolds could be used for the construction of tissue engineered heart valves.
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DOI：
10.1002/1097-4636(200105)55:2
发表时间：
2001-05
期刊：
Journal of biomedical materials research
影响因子：
--
作者：
D. W. Hutmacher;Thorsten Schantz;I. Zein;K. Ng;S. Teoh;K. C. Tan
通讯作者：
D. W. Hutmacher;Thorsten Schantz;I. Zein;K. Ng;S. Teoh;K. C. Tan
影响因子：
37.8
作者：
D. Schmidt;A. Mol;C. Breymann;J. Achermann;Bernhard Odermatt;M. Gössi;S. Neuenschwander;R. Prêtre;M. Genoni;G. Zund;S. Hoerstrup
通讯作者：
D. Schmidt;A. Mol;C. Breymann;J. Achermann;Bernhard Odermatt;M. Gössi;S. Neuenschwander;R. Prêtre;M. Genoni;G. Zund;S. Hoerstrup
DOI：
10.1007/978-1-59745-443-8_17
发表时间：
2007
期刊：
Methods in molecular medicine
影响因子：
--
作者：
A. Lichtenberg;S. Cebotari;I. Tudorache;A. Hilfiker;A. Haverich
通讯作者：
A. Lichtenberg;S. Cebotari;I. Tudorache;A. Hilfiker;A. Haverich
影响因子：
14
作者：
U. Stock;K. Schenke-Layland
通讯作者：
U. Stock;K. Schenke-Layland
影响因子：
2.4
作者：
Jun Zhang;Hongxu Qi;Hongjun Wang;P. Hu;L. Ou;Shuhua Guo;Jing Li;Yongzhe Che;Yao-ting Yu;D. Kong
通讯作者：
Jun Zhang;Hongxu Qi;Hongjun Wang;P. Hu;L. Ou;Shuhua Guo;Jing Li;Yongzhe Che;Yao-ting Yu;D. Kong