Synergy-directed fractionation of botanical medicines: a case study with goldenseal (Hydrastis canadensis).
Synergy-directed fractionation of botanical medicines: a case study with goldenseal (Hydrastis canadensis).
复制标题
DOI：
10.1021/np200336g
复制
发表时间：
2011-07-22
影响因子：
5.1
通讯作者：
Cech NB
中科院分区：
文献类型：
--
作者：
Junio HA;Sy-Cordero AA;Ettefagh KA;Burns JT;Micko KT;Graf TN;Richter SJ;Cannon RE;Oberlies NH;Cech NB
It is often argued that the efficacy of herbal medicines is a result of the combined action of multiple constituents that work synergistically or additively. Determining the bioactive constituents in these mixtures poses a significant challenge. We have developed an approach to address this challenge, synergy directed fractionation, which combines comprehensive mass spectrometry profiling with synergy assays and natural products isolation. The applicability of synergy directed fractionation was demonstrated using the botanical medicine goldenseal (Hydrastis canadensis) as a case study. Three synergists from goldenseal were identified, sideroxylin (1), 8-desmethyl-sideroxylin (2), and 6-desmethyl-sideroxylin (3). These flavonoids synergistically enhance the antimicrobial activity of the alkaloid berberine (also a constituent of H. canadensis) against Staphylococcus aureus by inhibition of the NorA multidrug resistance pump. The flavonoids possess no inherent antimicrobial activity against S. aureus; therefore, they could have been missed using traditional bioactivity directed fractionation. The flavonoid synergists are present at higher concentration in extracts from H. canadensis leaves, while the antimicrobial alkaloid berberine is present at higher levels in H. canadensis roots. Thus, it may be possible to produce an extract with optimal activity against S. aureus using a combination of goldenseal roots and leaves.
登录
查看更多内容
影响因子：
--
作者：
Noskin, GA;Rubin, RJ;Gemmen, E
通讯作者：
Gemmen, E
影响因子：
3.7
作者：
NOVICK, R
通讯作者：
NOVICK, R
DOI：
10.1016/s0924-8579(02)00162-0
发表时间：
2002-09-01
影响因子：
10.8
作者：
Price, CTD;Kaatz, GW;Gustafson, JE
通讯作者：
Gustafson, JE
影响因子：
5.1
作者：
Gu, JQ;Graf, TN;Oberlies, NH
通讯作者：
Oberlies, NH
影响因子：
2.7
作者：
Stermitz, FR;Scriven, LN;Lewis, K
通讯作者：
Lewis, K