Rapid detection of colistin resistance in Acinetobacter baumannii using MALDI-TOF-based lipidomics on intact bacteria.
Rapid detection of colistin resistance in Acinetobacter baumannii using MALDI-TOF-based lipidomics on intact bacteria.
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DOI：
10.1038/s41598-018-35041-y
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发表时间：
2018-11-15
影响因子：
4.6
通讯作者：
Larrouy-Maumus G
中科院分区：
文献类型：
--
作者：
Dortet L;Potron A;Bonnin RA;Plesiat P;Naas T;Filloux A;Larrouy-Maumus G
随着极端耐药细菌的传播，粘菌素现在被认为是治疗革兰氏阴性杆菌（包括产碳青霉烯酶菌）引起的感染的最后手段。不幸的是，粘菌素的使用增加也导致了耐药性的出现。以.在鲍曼不动杆菌中，粘菌素抗性主要是由通过pmrC基因染色体编码的磷酸乙醇胺转移酶的作用将磷酸乙醇胺添加到脂质A中引起的，所述pmrC基因由双组分系统PmrA/PmrB调节。以.鲍曼不动杆菌临床分离株对粘菌素的主要耐药机制涉及pmrA、pmrB或pmrC基因的突变，导致pmrC的过表达。尽管快速检测耐药性是提高感染患者治疗水平的关键问题之一，但A.鲍曼不动杆菌仍然依赖于通过微量稀释测定MIC，这是耗时的（16 - 24小时）。在这里，我们评估了最近描述的基于MALDI-TOF的检测方法MALDixin检测的性能，该检测方法可以快速检测粘菌素耐药相关的脂质A修饰（即磷酸乙醇胺添加）。该试验准确检测了所有粘菌素耐药A。在MALDI-TOF分析之前，使用非常有限的样品制备，在不到15分钟的时间内直接在完整的细菌上检测鲍曼不动杆菌分离株。
With the dissemination of extremely drug resistant bacteria, colistin is now considered as the last-resort therapy for the treatment of infection caused by Gram-negative bacilli (including carbapenemase producers). Unfortunately, the increase use of colistin has resulted in the emergence of resistance as well. In A. baumannii, colistin resistance is mostly caused by the addition of phosphoethanolamine to the lipid A through the action of a phosphoethanolamine transferase chromosomally-encoded by the pmrC gene, which is regulated by the two-component system PmrA/PmrB. In A. baumannii clinical isolate the main resistance mechanism to colistin involves mutations in pmrA, pmrB or pmrC genes leading to the overexpression of pmrC. Although, rapid detection of resistance is one of the key issues to improve the treatment of infected patient, detection of colistin resistance in A. baumannii still relies on MIC determination through microdilution, which is time-consuming (16–24 h). Here, we evaluated the performance of a recently described MALDI-TOF-based assay, the MALDIxin test, which allows the rapid detection of colistin resistance-related modifications to lipid A (i.e phosphoethanolamine addition). This test accurately detected all colistin-resistant A. baumannii isolates in less than 15 minutes, directly on intact bacteria with a very limited sample preparation prior MALDI-TOF analysis.
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