OsLAP6/OsPKS1, an orthologue of Arabidopsis PKSA/LAP6, is critical for proper pollen exine formation.
OsLAP6/OsPKS1, an orthologue of Arabidopsis PKSA/LAP6, is critical for proper pollen exine formation.
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OsLAP6/OsPKS1 是拟南芥 PKSA/LAP6 的直系同源物，对于正确的花粉外壁形成至关重要
DOI：
10.1186/s12284-017-0191-0
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发表时间：
2017-12-28
期刊：
影响因子：
--
通讯作者：
Li S
中科院分区：
文献类型：
--
作者：
Zou T;Xiao Q;Li W;Luo T;Yuan G;He Z;Liu M;Li Q;Xu P;Zhu J;Liang Y;Deng Q;Wang S;Zheng A;Wang L;Li P;Li S
背景：雄性育性对于水稻产量至关重要，而水稻产量的提高需要依靠雄性不育系的杂交生产。尽管最近的研究揭示了雄性生殖发育中的几个重要基因，但我们对水稻花粉发育机制的理解仍不清楚。结果：我们鉴定了一个由花粉外壁形成缺陷引起的具有完全雄性不育表型的水稻突变体oslap6。通过使用MutMap方法，我们发现位于OsLAP6/OsPKS1第二外显子的单核苷酸多态性（SNP）变异导致了突变表型。 OsLAP6/OsPKS1 是拟南芥 PKSA/LAP6 的直系同源基因，在孢粉质代谢中发挥作用。由CRISPR/Cas9基因组编辑工具产生的其他几个OsLAP6/OsPKS1功能丧失突变体也表现出相同的雄性不育表型。我们的细胞分析表明 OsLAP6/OsPKS1 可能通过影响杆状细胞伸长来调节花粉外壁的形成。表达检测表明OsLAP6/OsPKS1在绒毡层中特异表达，其产物定位于内质网(ER)。蛋白质序列分析表明OsLAP6/OsPKS1在陆地植物中是保守的。结论：OsLAP6/OsPKS1参与陆地植物中保守的孢粉素前体生物合成途径，是水稻雄性育性的关键分子开关。 OsLAP6/OsPKS1 的操作在杂交水稻育种中具有应用潜力。
Background:Male fertility is crucial for rice yield, and the improvement of rice yield requires hybrid production that depends on male sterile lines. Although recent studies have revealed several important genes in male reproductive development, our understanding of the mechanisms of rice pollen development remains unclear.Results:We identified a rice mutant oslap6 with complete male sterile phenotype caused by defects in pollen exine formation. By using the MutMap method, we found that a single nucleotide polymorphism (SNP) variation located in the second exon of OsLAP6/OsPKS1 was responsible for the mutant phenotype. OsLAP6/OsPKS1 is an orthologous gene of Arabidopsis PKSA/LAP6, which functions in sporopollenin metabolism. Several other loss-of-function mutants of OsLAP6/OsPKS1 generated by the CRISPR/Cas9 genomic editing tool also exhibited the same phenotype of male sterility. Our cellular analysis suggested that OsLAP6/OsPKS1 might regulate pollen exine formation by affecting bacula elongation. Expression examination indicated that OsLAP6/OsPKS1 is specifically expressed in tapetum, and its product is localized to the endoplasmic reticulum (ER). Protein sequence analysis indicated that OsLAP6/OsPKS1 is conserved in land plants.Conclusions:OsLAP6/OsPKS1 is a critical molecular switch for rice male fertility by participating in a conserved sporopollenin precursor biosynthetic pathway in land plants. Manipulation of OsLAP6/OsPKS1 has potential for application in hybrid rice breeding.
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