Integrated Surveillance for Antimicrobial Resistance in Salmonella From Clinical and Retail Meat Sources Reveals Genetically Related Isolates Harboring Quinolone- and Ceftriaxone-Resistant Determinants.
Integrated Surveillance for Antimicrobial Resistance in Salmonella From Clinical and Retail Meat Sources Reveals Genetically Related Isolates Harboring Quinolone- and Ceftriaxone-Resistant Determinants.
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DOI：
10.1093/ofid/ofab213
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发表时间：
2021-08
影响因子：
4.2
通讯作者：
Tewari D
中科院分区：
文献类型：
--
作者：
M'ikanatha NM;Yin X;Boktor SW;Dettinger LA;Tewari D
包括非伤寒沙门氏菌（NTS）在内的食源性病原体的抗微生物药物耐药性是一个公共卫生问题。宾夕法尼亚州对来自人类和动物的NTS的抗菌素耐药性进行综合监测。2015-2017年期间，临床实验室提交了4478株人类NTS分离株，在2520份零售肉类样本中发现了96株NTS分离株。109株临床分离株与肉品分离株及肉品样品中所有菌株具有相同的脉冲场凝胶电泳图谱，对抗菌药物进行了敏感性检测。采用全基因组测序（WGS）对6株临床分离株和96株肉源分离株（共102株）进行分析。109株临床NTS中28株（25.7%）和21株（21.9%）对3种以上抗菌药物耐药（多药耐药）。WGS分析的102株中有16株（15.7%）具有耐药机制，可对头孢曲松等广谱头孢菌素产生耐药。我们在2株临床分离的S. infant分离株和3株肉源分离的S. infant分离株中鉴定出blaCTX-M-65。这5株blactx - m -65阳性的婴儿链球菌携带了≥5个额外的耐药基因，加上gyrA中编码氟喹诺酮类药物耐药的D87Y突变。WGS结果显示，从患者和肉类分离的S. Infantis和S. Reading分别在≤10和≤5个等位基因范围内。很大一部分来自人类和动物的NTS分离株具有多重耐药，16%具有赋予头孢曲松耐药的遗传机制。这些结果强调了在卫生保健和农业环境中进行综合监测的必要性。
Antimicrobial resistance in foodborne pathogens, including nontyphoidal Salmonella (NTS), is a public health concern. Pennsylvania conducts integrated surveillance for antimicrobial resistance in NTS from human and animal sources. During 2015–2017, clinical laboratories submitted 4478 NTS isolates from humans and 96 isolates were found in 2520 retail meat samples. One hundred nine clinical isolates that shared pulsed-field gel electrophoresis patterns with meat isolates and all strains from meat samples were tested for susceptibility to antimicrobial agents. Six clinical and 96 NTS isolates from meat sources (total 102) were analyzed by whole-genome sequencing (WGS). Twenty-eight (25.7%) of the 109 clinical NTS and 21 (21.9%) of strains from meat sources had resistance to ≥3 antimicrobial drug classes (multidrug resistance). Sixteen of the 102 (15.7%) isolates analyzed by WGS had resistance mechanisms that confer resistance to expanded-spectrum cephalosporins, such as ceftriaxone. We identified blaCTX-M-65 in 2 S. Infantis isolates from clinical and 3 S. Infantis isolates from meat sources. These 5 blaCTX-M-65–positive S. Infantis strains carried ≥5 additional resistance genes plus a D87Y mutation in gyrA that encodes fluoroquinolone resistance. WGS showed that isolates from patients and meat samples were within ≤10 and ≤5 alleles for S. Infantis and S. Reading, respectively. A significant proportion of NTS isolates from human and animal sources were multidrug resistant and 16% had genetic mechanisms that confer resistant to ceftriaxone. These results emphasize need for integrated surveillance in healthcare and agricultural settings.
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影响因子：
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作者：
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